Product Name ANFITECH BIOTIN Synthetic Antibody Aptamer specific for Rat Brain Tumor Microvessels
Concentration 2 mg/ml
High affinity Oligonucleotide specific for Rat Brain Tumor Microvessels Background
Neoangiogenesis, the new formation of blood vessels is associated with endothelial cell proliferation, migration, and formation of new capillaries as a response to the increased demand of tumor tissue for oxygen and nutrients. In normal physiological processes, such as wound-healing, angiogenesis is tightly balanced by positive and negative regulators. In several disease states, such as tumor growth, overactive angiogenesis contributes to advancement of disease. Positive regulators of angiogenesis are recruited by the tumor to dominate negative regulators to ensure proliferation and organization of microvessel-forming. Specificity
Form & Buffer
Supplied as a liquid in sterilized high pure H20. Store at room temperature for short term storage. Aliquot and store at -20 deg C for long term storage. Applications
- Western blot - ELISA - Turbidimetry - Histochemistry - Affinity chromatography - Lateral Flow Assay Grade & Purity > 99% pure by SDS PAGE
Journal: THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 276, No. 19, Issue of May 11, pp. 16464–16468, 2001. Title: Systematic Evolution of a DNA Aptamer Binding to Rat Brain Tumor Microvessels - SELECTIVE TARGETING OF ENDOTHELIAL REGULATORY PROTEIN PIGPEN. Authors: Michael Blank‡§, Toni Weinschenk¶, Martin Priemeri, and Hermann Schluesener‡. Institutions: From the ‡Institute of Brain Research, University of Tuebingen, Calwer Strasse 3, D-72076 Tuebingen, Germany, the Institute for Cell Biology, Department of Immunology, University of Tuebingen, Auf der Morgenstelle 15, D-72076 Tuebingen, Germany, and the iInstitute for Cell Biology, Department of Molecular Biology, University of Tuebingen, Auf der Morgenstelle 15, D-72076 Tuebingen, Germany. Abstract: Tumor microvessels differ in structure and metabolic function from normal vasculature, and neoangiogenesis is associated with quantitative and qualitative changes in expression of endothelial proteins. Such molecules could serve as molecular addresses differentiating the tumor vasculature from those of the normal brain. We have applied Systematic Evolution of Ligands by Exponential enrichment (SELEX) against transformed endothelial cells as a complex target to select single-stranded DNA-ligands (aptamers) that function as histological markers to detect microvessels of rat experimental glioma, a fatal brain tumor that is highly vascularized. Both the SELEX selection procedure as well as subsequent deconvolution-SELEX were analyzed by fluorescence based methods (flow cytometry and fluorescence microscopy). Of 25 aptamers analyzed, one aptamer was selected that selectively bound microvessels of rat brain glioblastoma but not the vasculature of the normal rat brain including peritumoral areas. The molecular target protein of aptamer III.1 was isolated from endothelial cells by ligand-mediated magnetic DNA affinity purification. This protein was identified by mass spectrometry as rat homologue of mouse pigpen, a not widely known endothelial protein the expression of which parallels the transition from quiescent to angiogenic phenotypes in vitro. Because neoangiogenesis, the formation of new blood vessels, is a key feature of tumor development, the presented aptamer can be used as a probe to analyze pathological angiogenesis of glioblastoma. The presented data show that pigpen is highly expressed in tumor microvessels of experimental rat brain glioblastoma and may play an important role in warranting blood supply, thus growth of brain tumors.


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