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Cm-cat-innenleben 080201-enGeneral product description
Dehydrated Culture Media are specific preparations in form of powder. To prepare culture medium, a definite quantity of this powder has to be dissolved in distilled water, boiled and than sterilized. Culture media are liquid (i.e. broth media) or solid (i.e. agar media). Culture media are combinations of complex nutrients formulated for the cultivation of a more or less specific kind of micro-organism. The specificity of a culture medium is determined by its ingredients. Ingredients have to satisfy the nutritional requirements for growth and maintenance of the micro-organisms. Nutritional requirements as sources of energy, nitrogen, carbon, and trace elements are sat-isfied by carbohydrates, peptones, hydrolysates, extracts and infusions. Selective agents allow to select some micro-organisms that are resistant to it, by suppressing the sensi-tive ones. Selective agents are for instance bile salts or antibiotics . Other ingredients allow to identify the presence of certain micro-organisms, by making visible a specific biochemical characteristic. The micro-organisms can be distinguished by the colour of the colonies, by the colour of the media or other. For instance pH-indicators can reveal the formation of acids due to fermentation of a carbohydrate (which can be specific to a certain group of micro-organisms) by chang-ing the colour of the media. The oxidation-reduction indicators adopt particular colourations in their oxidized state, when there is oxygen present in the medium. For instance methylene blue changes from colourless to blue, and resazurin, from colourless to pink. Some other ingredients are buffering substances (e.g. phosphate salts), solidifying substances (e.g. agar), substances that maintain the osmotic pressure (e.g. sodium chloride) or growth factors. Applications
There are various applications of Dehydrated Culture Media which can be summarized as follows: isolation and enumeration of micro-organisms in cultures and subcultures, isolation and enumeration of micro-organisms in food and beverage, dairy products, pharmaceu-tical products, water and waste water or cosmetics, isolation of pathogenic micro-organisms that cause infections, identification of micro-organisms for therapeutic, epidemiological and taxonomic purposes, determination of the sensitivity of micro-organisms to antimicrobial agents, study of the biochemical and physiological characteristics of a micro-organism, evaluation of the biological activity of pharmaceutical preparations, Instructions for use
Dissolving the medium
Weigh the required amount of dehydrated culture medium, supplements and other preparations. Transfer all heat-stable ingredients to a clean and undamaged glass container (e.g. beaker). Attention: This glass container has to be at least twice as large as the final volume of medium to be obtained. Add a part of the distilled or de-ionized water necessary to obtained the final volume of medium and swirl the container thoroughly until all ingredients are dissolved. Add more distilled or de-ionized water to the container until the required volume has been reached. Heat the medium to boiling with frequent agitation to prevent overheating of the medium. This proce-dure often determines the clarity and performance of the finished product. pH adjustment
The pH value of the dehydrated culture medium shall produce the equivalent value at 25 °C as men-tioned in the product insert. If the pH value is not identical to the required value, adjust it with 0,1N HCl or NaOH solution. Sterilization
Most culture media require a sterilization in an autoclave. Sterilization is generally done at 121 °C for 15 minutes. The user should be aware that larger volumes take longer to heat up to the required steriliza-tion temperature. This has to be taken into account when running the sterilization process. Not all the prepared culture media are sterilized by autoclaving. Please follow the instructions on the product insert. Dispensing of the medium
Cool the agar based medium to approximately 45 - 50 °C. If any heat sensitive enrichments and supple-
ments are required they have to be added now to the medium. These sterile supplements must be
brought to room temperature before they are added to the medium.
Sterile broths may be cooled to room temperatures before addition of the supplements.
Dispense the medium into the sterile Petri dishes or tubes and immediately close them. Allow the agar based media to solidify for 1-2 hours. To prepare blood agar, defibrinated blood is recommended rather than blood containing an anticoagu-lant. Sterile blood should be stored at 2 - 8 °C, and brought to 35 - 37 °C before being added to the me-dium. Storage of prepared media
If the prepared medium will not be used on the same day it should be stored at a low temperature (2 - 8 °C) in moisture proofed containers. Petri dishes should be inverted. The caps on the tubes should be tightened firmly before storage. Disposal of medium
All samples and cultures should be handled carefully. After use, prepared plates, samples, sample con-tainers and other contaminated materials must be sterilized or incinerated before discarding. State and local environmental regulations have to be followed when disposing bio-hazardous materials. Precautions for use
Read the instructions on the product inserts carefully. Verify the expiration date and confirm the appearance of the media. Store dehydrated culture media at a dry place and at required temperature as stated on the product la-bel. Ensure proper capping of the box after use. Humidity may have adverse effects on the product. Efficiency of the sterilization process should be controlled. This can be achieved by thermo-electrical and/or biological validation. Only sterile laboratory ware as Petri dishes, test tubes etc should be used. Do not store prepared medium below 0 °C. Bring the stored media to room temperature before use. When ordering please remember to add the necessary supplements to your order of dehydrated media. Product listing
The product listing is devided into three parts: „Dehydrated Culture Media“ are listed on pp 4. „ Dehydrated Culture Media Supplements“ can be found on page 7. All other Laboratory Preparations are introduced on page 8. In order to facilitate the selection of a suitable culture medium we state the applicable national or inter-national standards for each product. An explanation of the acronyms used can be found below. AFNOR Association Française de Normalisation Dehydrated Culture Media
A medium with added supplements for selective isolation and enumeration of coagulase positive Staphylococcus species from Supplement : Egg Yolk Tellurite Emulsion A highly selective medium for isolation of Salmonella species, parti- CE IVD cularly S. typhi from clinical and non-clinical specimens. A non-selective general-purpose medium to which blood may be added for use in isolation and cultivation of Streptococcus species and other fastidious pathogenic organisms like Neisseria, etc. and also for detection of haemolytic activity. A highly nutritious general-purpose liquid medium for cultivation of a variety of fastidious and non-fastidious microorganisms, inclu-ding aerobic and anaerobic bacteria from a variety of clinical and non-clinical specimens. A medium for selective isolation of Salmonella species other than S. CE IVD typhi from clinical and non-clinical samples. A medium for detection of coliforms in water and waste water, food, milk and other dairy products as well as in other materials of sanitary importance. A selective medium for isolation of Pseudomonas aeruginosa from DCM-C02 Chloramphenicol Yeast Glucose Agar A selective medium for isolation and enumeration of yeast and moulds in milk, dairy products and other foodstuffs A basal medium for preparation of blood and chocolate agar and for various selective and identification media in isolating and culti- CE IVD vating fastidious micro-organisms. A selective medium for isolation of enteric pathogens particularly Salmonella and Shigella species. A differential and slightly selective medium for detection of coli-forms and other enteric micro-organisms. DCM-E02 Enterobacteriaceae Enrichment Broth A selective medium for selective enrichment of Enterobacteria- Added with Fraser Listeria Supplements, it forms a selective medi- um for enrichment and detection of Listeria species in food and A selective medium for isolation, enumeration and cultivation of A medium for the detection of coliforms, as a pre-enrichment broth for Salmonellae and in the study of lactose fermentation in general. A medium for detection of coliform bacteria in materials of sanita-ry importance. A slightly selective and differential medium for the detection of A slightly selective and differential medium for the presumptive identification of coliforms in water, milk and food. DCM-M03 Mannitol Salt Agar (Chapman Agar) A selective medium for isolation and identification of Staphylococ-cus aureus from clinical and non-clinical specimens. Palcam Listeria Identification Agar Base Added with Palcam Listeria Supplement it forms a selective medi-um for isolation and identification of Listeria species. A medium for obtaining microbial plate counts from milk and dai-ry products, foods, water and other materials of sanitary importan-ce. A non-selective medium for the cultivation and enumeration of yeasts and moulds from dairy products, foods and pharmaceutical preparations. A non-selective medium for total count of heterotrophic micro-organisms in drinking water. A selective enrichment broth for Salmonella species in foods and other materials. A non-selective medium for cultivation and enrichment of Clostri-dia and other anaerobes. A medium for selective cultivation of yeasts and moulds. A general purpose medium for the cultivation of yeasts, moulds and aciduric bacteria. A general purpose medium for the cultivation of yeasts, moulds and aciduric bacteria. A differential and selective medium for isolation of Salmonella and IVD CE some Shigella species from clinical and non-clinical specimens. An enrichment medium for the isolation of Salmonella species from faeces, urine, water, foods and other materials of sanitary importance. A medium for sterility testing of biologicals and cultivation of ae- A medium for identification of gram negative enteric bacilli on the AOAC basis of dextrose, lactose and sucrose fermentation and hydrogen sulphide production. A general purpose medium for the isolation and cultivation of a wide variety of fastidious and non-fastidious micro-organisms. A general purpose medium for the cultivation of a wide variety of fastidious and non-fastidious micro-organisms. A selective medium for the detection and enumeration of coliforms. A selective medium for detection and enumeration of Enterobacte- According to Mossel riaceae. A medium for cultivation and enumeration of yeasts A moderately selective medium for isolation and differentiation of AOAC Salmonella and Shigella species. A highly nutritive medium for cultivation of a wide variety of bac-teria. Yeast Glucose Chloramphenicol Agar (YGC) Dehydrated Culture Media Supplements
A sterile, stabilized emulsion of egg yolk recommended for use in culture media. A sterile, stabilized tellurite emulsion of egg yolk recommen-ded for identification of Staphylococcus species. An antibiotic supplement recommended for the selective isolation and identification of Listeria species. Sufficient for 500 ml prepared Fraser Broth A supplement recommended for the differentiation of Liste- Sufficient for 500 ml prepared Fraser Broth An antibiotic supplement that inhibits the growth of gram- An antibiotic supplement recommended for the selective isolation and identification of Listeria species. Sufficient for 500 ml prepared Palcam Listeria Identification Agar Other Laboratory Preparations
For use as a solidifying agent in microbiological culture me-dia. An ingredient used in the preparation of variety of culture media such as sterility testing media, diagnostic media and media for biochemical characterization. An ideal ingredient used in the preparation of culture media for the cultivation of yeasts and moulds. Papaic digest of soybean meal, used in the preparation of culture media for the cultivation of many fastidious micro-organisms, including fungi. A nutritious extract used in the preparation of culture media for the cultivation of a wide variety of micro-organisms. Applications
DCM-R02 Rappaport Vassiliadis Soya Broth P. aeruginosa
Yeasts and Moulds
Food and Beverages
DCM-E02 Enterobacteriaceae Enrichment Broth Enterobacteriaceae DCM-T02 Triple Sugar Iron Agar
DCM-R02 Rappaport Vassiliadis Soya Broth Salmonella sp.
Fraser Enrichment Broth Base + Supplements Selective Enrichment Listeria sp.
DCM-P01 Palcam Listeria Identification Agar Base + Selective Isolation Yeasts and Mould
Medium base for blood and chocolate agar General Purpose
Enterobacteriaceae DCM-T02 Triple Sugar Iron Agar
Yeasts and Mould
DCM-P01 Palcam Listeria Identification Agar Non-selective enrichment and cultivation General Purpose
Water and Waste Water
Selective Enrichment and MPN Presumptive Staphylococcus sp. SP-E02
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