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Intrahepatic cholestasis of pregnancy: a randomized controlled trial comparing dexamethasone and ursodeoxycholic acidIntrahepatic Cholestasis of Pregnancy: a Randomized Controlled Trial Comparing Dexamethasone and Anna Glantz,1 Hanns-Ulrich Marschall,2 Frank Lammert,3 Lars-Åke Mattsson1 Intrahepatic cholestasis of pregnancy (ICP) is characterized by troublesome maternal pru-
ritus, elevated serum bile acids (>10 mol/L) and increased fetal risk. Recently we deter-
mined a cutoff level of serum bile acids, >40 mol/L, to be associated with impaired fetal
outcome. We have now studied the effects of ursodeoxycholic acid (UDCA) and dexameth-
asone on pruritus, biochemical markers of cholestasis, and fetal complication rates in a
double-blind, placebo-controlled trial. For this purpose, 130 women with ICP were ran-
domly allocated to UDCA (1 g/day for three weeks), or dexamethasone (12 mg/day for 1
week and placebo during weeks 2 and 3), or placebo for 3 weeks. Pruritus and biochemical
markers of cholestasis were analyzed at inclusion and after 3 weeks of treatment. Fetal
complications (spontaneous preterm delivery; asphyxial events; and meconium staining of
amniotic ﬂuid, placenta, and membranes) were registered at delivery. An intention-to-treat
analysis showed signiﬁcant reduction of alanine aminotransferase (ALT) (P ؍ .01) and
bilirubin (P ؍ .002) in the UDCA group only. In a subgroup analysis of ICP women with
serum bile acids >40 mol/L at inclusion (n ؍ 34), UDCA had signiﬁcant effects on
pruritus (؊75%), bile acids (؊79%), ALT (؊80%), and bilirubin (؊50%) as well, but not
on fetal complication rates. Dexamethasone yielded no alleviation of pruritus or reduction of
ALT and was less effective than UDCA at reducing bile acids and bilirubin. In conclusion, 3
weeks of UDCA treatment improved some biochemical markers of ICP irrespective of
disease severity, whereas signiﬁcant relief from pruritus and marked reduction of serum bile
acids were only found in patients with severe ICP. (HEPATOLOGY 2005;42:1399-1405.)
Intrahepatic cholestasis of pregnancy (ICP) is a tion.1 Although essentially benign from a maternal liver disease of as yet undeﬁned etiology and viewpoint, ICP is associated with increased fetal risks, pathogenesis. ICP is characterized by pruritus and such as preterm delivery in 19% to 60%,2-4 fetal distress in elevated serum bile acids (Ն10 mol/L) with onset in the 22%-41%,3,5-8 and intrauterine fetal death in 1% to 4% second half of pregnancy and persisting until delivery.
of affected pregnancies.3,6,9 Fetal complication rates are Family clustering and varying incidence in different geo- related to maternal serum bile acid levels, and increase graphic regions suggest an underlying genetic explana- when bile acid levels exceed 40 mol/L.10 Previous stud-ies have indicated that it is possible to reduce pruritus andsome surrogate parameters of cholestasis by pharmaco- Abbreviations: ICP, intrahepatic cholestasis of pregnancy; UDCA, ursodeoxy- logic treatment. Agents such as phenobarbital, anion ex- cholic acid; ALT, alanine aminotransferase; ALP, alkaline phosphatase; ITT, in- changers, S-adenosyl methionine, and others have been From the Department of Obstetrics and Gynecology, Sahlgrenska University tested but not introduced into clinical practice due to Hospital/East, Go¨teborg, Sweden; Department of Medicine, Karolinska University uncertain efﬁcacy and/or intolerable side effects.11-13 Hospital Huddinge, Stockholm, Sweden; and Department of Internal Medicine I,University Hospital Bonn, Bonn, Germany. However, small studies with ursodeoxycholic acid Received July 26, 2005; accepted September 19, 2005. (UDCA) and dexamethasone indicate that pharmaco- Supported by grants from FoU, Va¨stra Go¨taland. logic treatment may provide relief from pruritus and re- Address reprint requests to: Anna Glantz, M.D., Department of Obstetrics and Gynecology, Sahlgrenska University Hospital/East, 416 85 Go¨teborg, Sweden. E- duce serum bile acid and liver enzyme levels. UDCA has mail: email@example.com; fax: (46) 31 25 43 87. been tested in two randomized, placebo-controlled stud- Copyright 2005 by the American Association for the Study of Liver Diseases. ies, with 8 patients in each group.14,15 The effects of dexa- Published online in Wiley InterScience (www.interscience.wiley.com).
DOI 10.1002/hep.20952 methasone were investigated in an uncontrolled study of Potential conﬂict of interest: Nothing to report. 10 women.16 These studies were not able to prove the effectiveness of any of these treatments. Thus, we per- capsules to be ﬁlled with dexamethasone, all of identical formed a randomized, placebo-controlled trial aiming at appearance, were supplied by Dr. Falk Pharma (Freiburg, comparing the effects of UDCA and dexamethasone on: Germany). The hospital pharmacy at Sahlgrenska Uni- (i) alleviation of pruritus, (ii) improvement in serum liver versity Hospital, Go¨teborg, ﬁlled the empty capsules with tests, and (iii) the possible reduction of fetal complication dexamethasone, packed study drugs in anonymous tins rates after a treatment period of 3 weeks in women with marked with a study code, and was responsible for ran- domization. The study drugs were randomized in blocksof 6 treatments, containing 2 each of UDCA, dexameth- Patients and Methods
asone, and placebo. Staff at the 6 participating centers Between February 1, 1999, and January 31, 2002, all were instructed to hand out the treatments consecutively, pregnant women in the Va¨stra Go¨taland region of Swe- starting with the lowest study code number. When all 6 den were prospectively screened for ICP, deﬁned as oth- treatments in the block were dispensed or when 6 months erwise unexplained pruritus of pregnancy combined with had elapsed, a new block was distributed.
fasting bile acid levels Ն10 mol/L. There were The primary outcome variable was the rate of the fol- 1,500,462 inhabitants in the region in February 2001, lowing fetal complications, registered at delivery and and 45,485 pregnancies leading to deliveries during the compared between groups: spontaneous preterm delivery study period. In Sweden, all pregnancies are monitored by (Ͻ37 weeks) in singleton pregnancies, asphyxial events midwives in local prenatal clinics. If complications occur, (operative delivery due to asphyxia, postpartum pH women are referred to the nearest department of obstet- Ͻ7.05 in umbilical arterial blood or Apgar score Ͻ7 at 5 rics for further care. All 106 prenatal clinics and all 6 minutes), and meconium staining of amniotic ﬂuid, pla- departments of obstetrics in the region participated in the centa, and membranes. Changes of biochemical markers and pruritus were secondary variables for evaluation. Dif- During the study period, 937 women (2.1%) com- ferences between groups regarding other obstetric vari- plained of generalized pruritus during pregnancy and ables such as total prematurity rate (including preterm were, after giving written consent, included in the obser- inductions of labor and preterm Caesarean sections), total vational part of the ICP study. A study protocol recording elective delivery rate (inductions of labor and planned data on heredity, prior pregnancies, skin disorders, aller- Caesareans), and maternal blood loss during vaginal de- gic conditions, and hepatic, biliary, and metabolic condi- tions was given to each patient. Puncture of an antecubital Blood samples were analyzed for bile acids, amin- vein was performed after a 12-hour fast to determine se- otransferases, and bilirubin at inclusion, after 2-3 days, rum bile acids on a weekly basis until parturition. If serum after 4-5 days and after 1, 2, and 3 weeks of treatment. If bile acids exceeded 10 mol/L at any time, the woman the pregnancy continued after completed treatment, the was diagnosed with ICP and referred to the nearest de- same variables were analyzed weekly until parturition.
partment of obstetrics for further care. ICP was detected Treatment effects on biochemical markers of cholesta- in 693 women (1.5%). The results of the observational sis and pruritus were evaluated as the differences between part of our ICP study have recently been published.10 values at inclusion and after 3 weeks of treatment. Total Women with ICP and a gestational age less than 37 serum bile acid concentrations were analyzed with an en- weeks were invited to participate in a double-blind, pla- zymatic, colorimetric method (Enzabile, Biostat Diagnos- cebo-controlled study comparing treatment effects of tic Systems, Stockport, UK). Aminotransferases and UDCA and dexamethasone. Exclusion criteria were dia- serum bilirubin levels were analyzed with standard labo- betes, pre-eclampsia, intrauterine growth restriction, liver ratory methods. Pruritus was estimated on a 100-mm– disease (including viral hepatitis), and history of manic long visual analog scale with the endpoints “no pruritus at disorders or bleeding gastric ulcer. After exclusion, 425 all” (0 mm) and “worst possible pruritus” (100 mm).
Fetal well-being was monitored by cardiotocography at Women who chose to participate were consecutively enrolled after giving written consent. In a double-blind The results of the serum analyses were open to the fashion, they were randomly allocated to 1 of 3 groups.
managing obstetricians, who were not given any speciﬁc The ﬁrst group received UDCA, 1 g/day as a single dose, instructions regarding timing of deliveries. The study was for 3 weeks. The second group was allocated to dexameth- approved by the Swedish Medical Products Agency and asone, 12 mg/day as a single dose for 1 week, and placebo the Ethics Committee of the Faculty of Medicine at the during weeks 2 and 3. The third group was given placebo University of Go¨teborg. All enrolled women gave written for 3 weeks. UDCA capsules, placebo capsules, and empty informed consent, according to the Helsinki declaration.
Table 1. Obstetric and Biochemical Characteristics at Inclusion
All included n ؍ 130
UDCA n ؍ 47
Placebo n ؍ 47
NOTE. Onset of pruritus, maternal and gestational age at inclusion, and biochemical variables are expressed as means Ϯ SD. Intensities of pruritus are expressed Statistical Analysis.
and bilirubin at inclusion did not differ between groups, medians, and ranges were calculated for descriptive pur- nor did median values of pruritus intensity (Table 1).
The majority of the women (96 of 130) had bile acid The Wilcoxon signed rank test was used for ordinal levels of 10-39 mol/L at inclusion. A severe form of ICP variables and for tests over time within groups. Changes with bile acid levels Ն40 mol/L was identiﬁed in 34 over time between all 3 groups were analyzed by the women (12 women in the UDCA group and in 11 Kruskal-Wallis test, and the Mann-Whitney U test was women in the dexamethasone and placebo groups, respec- used for pairwise comparisons. Comparisons of patient characteristics and fetal and maternal outcome were Treatment Effects on Maternal and Fetal Compli-
tested with the chi-square test or StudentЈs t test, as ap- cation Rates. Fetal and maternal complication rates are
propriate. P values less than .05 were considered statisti- presented in Table 2. The results are presented as inten- tion-to-treat (ITT) analysis (Table 2A) and as a subgroup Our estimation of the number of patients needed to analysis in women with severe ICP (Table 2B). Sponta- treat to observe differences in fetal complication rates be- neous preterm deliveries in singleton pregnancies and to- tween the UDCA, dexamethasone, and placebo groups tal prematurity rate and meconium staining of amniotic was based on fetal complication rates previously reported ﬂuid, placenta, and membranes were approximately dou- in the literature.2-9 Based on a possible drug-related re- bled in the severe ICP group, whereas the rates of asphyx- duction of the fetal complication rate by 33%, a signiﬁ- ial events and total elective delivery did not differ when cance level of 0.05, a two-tailed analysis, and a power of these parameters in the ITT group were compared with 80%, 240 patients (80 in each arm) were needed to treat.
those of women with severe ICP. Treatment with UDCA A subgroup analysis of changes in laboratory parame- or dexamethasone did not improve any outcome variable ters and pruritus during treatment was performed in ICP in the ITT analysis group or in the subgroup of women women with serum bile acids Ն40 mol/L at inclusion.
This analysis was based on the data from our recent ob- Treatment Effects on Biochemical Markers and
servational study10 in which complication rates did not Pruritus. Changes in bile acids, ALT, bilirubin, and
increase until serum bile acid values exceeded 40 mol/L.
pruritus during treatment with UDCA, dexamethasone,and placebo are presented in Fig. 1 as an ITT analysis including all women (ﬁlled lines) and as a subanalysis of A total of 130 women met the inclusion criteria and women with bile acid levels Ն40 mol/L at inclusion chose to participate in the treatment study. A total of 47 were randomized to UDCA, 36 to dexamethasone, and ITT analysis (n ϭ 130) revealed signiﬁcant reductions 47 to placebo. There were no differences between groups in ALT (Fig 1B, P ϭ .01) and bilirubin (Fig. 1C, P ϭ regarding parity or previous history of ICP. Mean values .002) in the UDCA group compared with the placebo (ϮSD) of pruritus onset; maternal and gestational age; group. Changes in bile acids and pruritus did not reach and levels of bile acids, alanine aminotransferase (ALT), statistical signiﬁcance. Overall, no signiﬁcant changes Table 2A. Fetal Complication Rates and Outcomes of Pregnancies: ITT Analysis
UDCA n ؍ 47
Placebo n ؍ 47
were revealed during treatment with dexamethasone or pruritus scores paralleled bile acid levels. Median pruritus score on a visual analog scale was 74 mm at inclusion, In women presenting with severe ICP (Ն40 mol/L) increasing to 79 mm after 2-3 days of treatment. There at inclusion (n ϭ 34), serum bile acid levels were reduced was a subsequent decrease to 72 mm after 4-5 days of by treatment both in the UDCA (Ϫ79%) and in the treatment and further to 55, 27, and 18 mm after 1, 2, dexamethasone (Ϫ45%) groups, compared with placebo and 3 weeks of treatment, respectively.
(ϩ13%) (P ϭ .001 and P ϭ .01, respectively). The re- Tolerability and Duration of Treatment. The du-
duction was more pronounced in the UDCA group than ration of treatment is illustrated in Table 3. A total of 80 in the dexamethasone group (P ϭ .001; Fig. 1A). In the women completed the 3-week treatment period. One UDCA group, serum bile acid levels increased from woman in each group discontinued because of side effects 101 Ϯ 58 mol/L to 153 Ϯ 111 mol/L during the ﬁrst within 3 days (UDCA, 1 patient after 3 days because of 2-3 days of treatment. After 4-5 days the values decreased diarrhea; dexamethasone, 1 patient after 2 days because of to 115 Ϯ 105 mol/L, and further to 83 Ϯ 77; 36 Ϯ 52; nausea, dizziness, and stomach pain; placebo, 1 patient and 21 Ϯ 13 mol/L after 1, 2, and 3 weeks of treatment, after 1 day because of severe headache). One woman in respectively. The initial decrease of bile acids in the dexa- each group was randomized to treatment but did not start methasone group was rapid but transient, from 71 Ϯ 22 medication because of fear of side effects. The remaining to 26 Ϯ 26 mol/L after the ﬁrst week of treatment, but women discontinued their treatment because of sponta- rising again to 39 Ϯ 22 mol/L after 3 weeks of treat- Adverse Events. One case of intrauterine fetal death
A reduction in ALT was only found in the UDCA occurred during the study period. The subject was a 26- group (UDCA vs. dexamethasone: P ϭ .002, UDCA vs.
year-old woman undergoing her ﬁrst pregnancy, who was placebo: P ϭ .01, Fig. 1B). A reduction in bilirubin was medicated with clomipramine, 10 mg daily, due to a long- observed both in the UDCA group and in the dexameth- term depressive disorder with anxiety and panic attacks.
asone groups vs. placebo (P Ͻ .001 and P ϭ .001, respec- Her pruritus started in week 33, and she was allocated to tively; Fig. 1C). Pruritus was alleviated only in the UDCA treatment in week 36. Bile acid levels were 16 mol/L group and differed signiﬁcantly from the dexamethasone both at inclusion and after 2 weeks of treatment. cardio- group (P ϭ .01) and the placebo group at the end of tocography was normal at check-up visits after 1 and 2 treatment (P ϭ .001; Fig. 1D). In the UDCA group, weeks of treatment. Two days after her last check-up, Table 2B. Fetal Complication Rates and Outcomes of Pregnancies: Subanalysis of ICP Women With Bile Acids >40 mol/L
All n ؍ 34 (%)
UDCA n ؍ 12 (%)
n ؍ 11 (%)
Placebo n ؍ 11 (%)
NOTE. Fetal complication rates and outcomes of pregnancies as an ITT analysis (A), and a subgroup analysis in women with bile acid levels Ն40 mol/L at inclusion *Spontaneous onset of labor Ͼ37 weeks in singleton pregnancies.
†Operative delivery due to asphyxia, arterial umbilical pH Ͼ7.05, or Apgar Score Ͻ7 at 5 minutes of age.
‡Including multiple pregnancies and iatrogenous prematurity.
§The sum of planned deliveries, including inductions of labor and caesarean sections.
Fig. 1. Mean levels of serum bile acids (A), ALT (B), bilirubin (C), and median of pruritus scores (D) in women with ICP at inclusion and after 3 weeks of treatment with ursodeoxycholic acid (UDCA), dexamethasone (dexa) and placebo. Filled lines represent results in the ITT group (n ϭ 130)and dotted lines show results in the subgroup analysis of women with severe ICP (Ն40 mol/L) at inclusion. (A) Serum bile acids mol/L; means).
No changes were observed after ITT analysis. In women with severe ICP at inclusion, both UDCA and dexamethasone reduced bile acids, comparedto placebo (UDCA vs. placebo: P ϭ .001, dexamethasone vs. placebo: P ϭ .01). UDCA was more effective than dexamethasone (UDCA vs.
dexamethasone: P ϭ .001). (B)Serum ALT (U/L; means). ITT analysis showed a reduction of ALT in the UDCA group vs. placebo (P ϭ .01). In womenwith severe ICP at inclusion, ALT was reduced only in the UDCA group (UDCA vs. dexamethasone: P ϭ .002; UDCA vs. placebo: P ϭ .01;dexamethasone vs. placebo: n.s.). (C) Serum bilirubin (mol/L; means). ITT showed a reduction of bilirubin in the UDCA group vs. placebo (P ϭ.002). In women with severe ICP at inclusion, bilirubin was reduced both in the UDCA and dexamethasone group vs. placebo (P Ͻ .001 and P ϭ.001, respectively), but UDCA was proven to be more effective than dexamethasone within the group over time (UDCA: P Ͻ .001, dexamethasone:P ϭ .04). (D) Pruritus score (visual analog scale, 0 mmϭno pruritus, 100 mmϭworst possible pruritus; medians). No changes were observed afterintent-to-treat analysis. In women with severe ICP at inclusion, UDCA was more effective than dexametasone (P ϭ .01) and placebo (P ϭ .001),and a reduction of pruritus within the group over time was only noticed in the UDCA group (UDCA: P ϭ .001; dexamethasone: n.s.; placebo: n.s.).
spontaneous labor began. Intrauterine fetal death was di- was unable to demonstrate differences between treatment agnosed in the delivery ward, and the patient delivered a groups regarding fetal complication rates. According to stillborn baby (birth weight, 3650 g). The amniotic ﬂuid power calculations based on previously published fetal was heavily meconium stained. No autopsy was per- complication rates in women with ICP, 240 women formed. The incident was reported to the Swedish Med- should have been included, 80 in each arm. Only 130 of ical Products Agency as an adverse event. When the study 425 eligible women, according to inclusion criteria, ac- code was broken, it was revealed that this woman had cepted participation. The most common explanation for not entering the study was fear of negative treatment ef-fects on the fetus, which may be the major obstacle to Discussion
clinical studies of pharmacologic agents in pregnant Intrahepatic cholestasis of pregnancy has been re- women, presumably not only in Sweden. It is noteworthy ported to be associated with maternal pruritus and in- that even if 240 women had been included, it would have creased fetal risk, including intrauterine fetal death.2-9 been insufﬁcient to reveal treatment effects on fetal out- This double-blind, placebo-controlled study was de- come because the overall number of fetal complications signed to compare the effects of UDCA and dexametha- was substantially lower in our ICP study population than sone on fetal outcome, biochemical markers of cholestasis, and maternal pruritus in women diagnosed However, because the observational part of our ICP with ICP. Based on an ITT analysis, the only observed study recently demonstrated that fetal complication rates signiﬁcant effects of any treatment were UDCA-induced do not increase until bile acid levels exceed 40 mol/L,10 reductions of serum bilirubin and ALT levels. The study the subgroup of women presenting with bile acid levels Ն40 mol/L at randomization was analyzed separately.
This subgroup analysis revealed that treatment with Table 3. Duration of Treatment
UDCA actually improved all biochemical markers of cho- Dexamethasone
lestasis as well as pruritus, and that treatment with UDCA P Value
was more effective than treatment with dexamethasone or placebo. Mean bile acid levels decreased by 79% in the UDCA group, compared with a 45% decrease in the dexamethasone group and a 13% increase in the placebogroup (UDCA vs. dexamethasone, P Duration of therapy in 3 groups of women with ICP, randomized to treatment with ursodeoxycholic acid (UDCA), dexamethasone, or placebo for 3 weeks.
placebo, P ϭ .001). In this subgroup analysis, the UDCA- induced reductions of ALT (by 80%) and bilirubin (by study medication was strictly double blind, and the ran- 50%) were more pronounced than in the ITT analysis domization procedure was performed by a professional and also differed signiﬁcantly from the ALT and bilirubin authority. However, boxes containing the study medica- reductions observed in the dexamethasone (ALT, Ϫ17%; tion had to be replaced at the study centers every 6 bilirubin, Ϫ22%) and placebo groups (ALT, Ϫ18%; bil- months, regardless of whether all study medications had irubin, Ϫ11%; for ALT, UDCA vs. dexamethasone: P ϭ been used or not. Some study centers did not include .002; UDCA vs, placebo: P ϭ .01). However, although more than 1 or 2 patients durinig every 6-month period the relative incidence of a number of fetal complications of, leading to a large amount of study medication not such as preterm delivery, meconium passage and green- being used, which might have had an impact on the dif- staining of placenta/membranes was approximately twice as high in women with bile acids exceeding 40 mol/L In this study, both UDCA and dexamethasone were compared with women with bile acid levels Ͻ40 mol/L well tolerated, with similar discontinuation rates. The rea- (Table 2), the subanalysis did not reveal any difference in son for choosing only 1 week of active treatment followed the outcome related to treatment with UDCA or dexa- by 2 weeks of placebo in the dexamethasone group was that this is the only dose regimen of dexamethasone treat- The efﬁcacy of UDCA in ICP pregnancies has been ment in ICP pregnancies previously reported in the liter- previously investigated in 2 small randomized, placebo- ature.16 Furthermore, questions have recently been raised controlled ICP trials, both with 8 patients in the UDCA concerning potentially harmful effects of corticosteroids group and treatment periods of 20 days. One study on the developing fetal brain. This has lead to a more showed a reduction of all investigated variables (pruritus, restrictive approach to corticosteroid treatment during bile acids, bilirubin, and alkaline phosphatase [ALP]) in pregnancy,17,18 The data from the present study showed the UDCA group, but also a signiﬁcant reduction in pru- that dexamethasone was less effective than UDCA regard- ritus and ALP in the placebo group.14 The other study ing all studied variables. Therefore we conclude that showed a decrease of aminotransferases, bilirubin, and UDCA is currently the best pharmacologic treatment op- pruritus in the UDCA group, but no reduction of bile acids,15 which is in line with the results of our study.
Proof of the efﬁcacy of UDCA, in particular the effect Efﬁcacy in reducing maternal serum bile acid levels seems on fetal complication rates, nevertheless remains to be to be an important quality when choosing a drug for the elicited from investigations of an even larger study popu- treatment of ICP because bile acid levels have been dem- lation. We propose a serum bile acid level of Ն40 mol/L onstrated to have a dose-dependent association with fetal as the lower limit for inclusion of patients.
complication rates.10 In conﬂict with a previous report on In conclusion, ITT analysis showed that UDCA reduced dexamethasone therapy in ICP women,16 we found the ALT and bilirubin, but not bile acids or pruritus, in women effects on pruritus and biochemical markers of cholestasis with ICP. In patients with bile acid levels exceeding 40 to be transient in the dexamethasone group. A rapid de- mol/L, treatment with UDCA was more effective in alle- crease in pruritus and bile acids was seen after the ﬁrst viating pruritus and reducing bile acids, aminotransferases, week of treatment, but was followed by an increase.
and bilirubin, compared with treatment with dexametha- An interesting detail was the observation of an increase sone and placebo. However, no differences in fetal or mater- of total serum bile acid levels during the ﬁrst 2-3 days of nal complication rates could be detected. Although this is as treatment in the UDCA group, decreasing close to base- yet the largest treatment study of ICP, the sample size was line after 4-5 days of treatment, after which a steady de- still too small to answer the important question of whether crease occurred. This phenomenon was interpreted as a fetal complications in ICP can be reduced by pharmacologic dose-dependent medication effect, initially adding supra- treatment. Nevertheless, our data may help to deﬁne inclu- physiological levels of UDCA to the total maternal bile sion criteria for an even larger, multicenter, double-blind, acid pool. Interestingly, the pruritus scores paralleled the placebo-controlled study of UDCA for the treatment of ICP serum bile acid levels, increasing in the ﬁrst days of treat- ment and then gradually decreasing. Thus, from a clinicalviewpoint, the efﬁcacy of UDCA treatment in bile acid level reduction in ICP cannot be evaluated earlier than wives in the Va¨stra Go¨taland region for enrolling and taking care of study patients. Study nurse Ann Christians- The fact that the randomized groups differed in size son was of invaluable help. Special thanks to Anders Ode´n (dexamethasone n ϭ 36, UDCA and placebo n ϭ 47, and Emma Jaensson for their help with the statistical cal- respectively) is surprising. The random allocation to the References
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Wilma Zanetti [firstname.lastname@example.org] ESTENOSE AÓRTICA - UM ESTUDO DE CASO A população idosa tem patologias freqüentes tais como diabetes, problemas neurológicos, hepatológicos, hematológicos, nefropatias e cardiovasculares dentre os quais está a estenose aórtica. As principais causas de estenose aórtica são a congênita, reumática e a degenerativa ou senil (RANGEL, 2006). A