Index_poster_iinovo.indd

Poster Sessions Abstracts Index
PEROXIDASE USING DIFFERENT METHODS OF CO- SPREADING IN LIPID FILMS AT THE AIR-WATER Symposium I
David Sotero do Santos Jr (Windsor University), Luciano Caseli (IFSC- USP), Thaís Fernandes Schmidt (IFSC-USP) and Osvaldo Novais de POSTER SESSION PI1- Wednesday October 11
The interaction of horseradish peroxidase (HRP), an enzyme that catalyzes the hydrogen peroxide hydrolysis, with Langmuir monolayers of lipids is investigated. Surface Pressure-Area (p-A) isotherms of HRP-dipalmitoylph I501 - COMPARATIVE STUDY BETWEEN RAW NATURAL osphatidylglycerol (DPPG) mixed monolayers were studied, by using several RUBBERS FROM TWO NEW CLONES OF IAC SERIES techniques of enzyme-lipid co-spreading. We have found that interaction of HRP with DPPG depends on the method of co-spreading, which reflects the Luiz Henrique Capparelli Mattoso (Embrapa Instrumentação mechanism and dynamic of enzyme incorporation into the lipid film at the Agropecuária), Aldo Eloizo Job (Departamento de Física, Química e Biologia - Faculdade de Ciências e Tecnologia - UNESP/P. Prudente), Paulo de Souza Gonçalves (Instituto Agronômico) and Rogério Manoel Biagi Moreno (Embrapa Instrumentação Agropecuária) The natural rubber from two new clones of Hevea brasiliensis and the commercial one was evaluated by TG-DTG and DMA to characterize the rubbers. The TG and DTG results indicated very small differences between Ruy Carlos Ruver Beck (UFSM), Adriana Raffin Pohlmann (UFRGS), the clones. DMA results suggest a possible variation in the average Cristiane de Bona da Silva (UFRGS), Aline Ferreira Ourique (UFSM), molecular mass of the polyisoprene and also a variation in its distribution Rossana Barcellos Friedrich (UFSM), Silvia Stanisçuaski Guterres (UFRGS) and Márcia Camponogara Fontana (UFSM) In this study we evaluate the use of polymeric nanocapsule suspensions as a binder vehicle for the preparation of granules containing nanocapsules as an intermediate pharmaceutical product. Dexamethasone-loaded nanocapsules were prepared by interfacial deposition of preformed polymer [poly(e- Carlos Angelo Nunes (EEL/USP), Sinara Borborema Gabriel (UFRJ) caprolactone)] using two different oily phases (capric/caprilic mixture or sunflower oil). They presented nanometric size (180-260 nm), negative zeta potential and encapsulation efficiency close to 90%. All binders studied This study investigated the elastic properties of the as-cast Ti-10Mo-xNb (x showed a good compatibility with these suspensions in the range of 0.5 and = 3, 6 and 9) alloys recently developed. The Young’s moduli were determined by ultrasonic-pulse method. The density of each alloy was measured by the Archimedes principle. The densities of Ti-10Mo-xNb alloys increased with the concentration of niobium. The moduli of the Ti10Mo-6Nb and Ti-10Mo-9Nb alloys were similar while the Young’s modulus of Ti-10Mo-3Nb was lower than those of Ti and Ti-6Al-4V. In conclusion, among the three alloys, the FLUOROALUMINOSILICATE BY A NON-HYDROLYTIC alloy Ti-10Mo-3Nb had lower density and modulus, so being a promising Lilian Rodrigues Avila (UNIFRAN), Katia Jorge Ciuffi (UNIFRAN), Paula Fabiana dos Santos Pereira (UNIFRAN), Paulo Sérgio Calefi (UNIFRAN), Eduardo José Nassar (UNIFRAN) and Alexandre Cestari (UNIFRAN) I503 - STUDY OF THE INTERACTION OF PHYTASE Calcium fluoroaluminosilicate glasses are used as restorative materials for WITH NANOSTRUCTURED LIPID FILMS BY HARMONIC dental applications. The nonhydrolytic sol-gel route allows for the production of inorganic polymers based on an Al-Si matrix. In this work, three different mass percentages of a glass composition were synthesized to improve Maria Elisabete Darbellho Zaniquelli (FFCLRP - USP), Thatyane physical and chemical characteristics of the cement. In conclusion, Morimoto Nobre (FFCLRP - USP), Maristela Ferreira (FCTPP - UNESP), these materials synthesized by the nonhydrolytic sol-gel route presented Ubirajara Pereira Rodrigues Filho (IQSC - USP), Vantencir Zucolotto structures similar to those of glass ionomer powders, as confirmed by our (IFSC - USP), Marli Leite Moraes (IFSC - USP), Luciano Caseli (IFSC - characterizations and literature references.
USP) and Osvaldo Novais Oliveira Junior (IFSC - USP) The interaction of phytase, an enzyme that catalyzes the phytic acid hydrolysis, with Langmuir and Langmuir-Blodgett films of lipids is investigated. Surface I507 - SOLID STATE NITRIC OXIDE SENSOR USING A tension and rheology studies showed that phytase interacts remarkably with lipid monolayers, increasing their dynamic surface elasticity and making it Carlos Alberto Brunello (FFCLRP), Rondinelli Donizetti Herculano feasible to transfer mixed monolayers onto solid supports. The process of (FFCLRP) and Carlos F. O. Graeff (FFCLRP) enzyme adsorption is quicker than the frequency imposed to the oscillating drop. It is likely that the relaxation process due to area variations should be The proposed sensor is a well known spin trap iron(II)-diethyldithiocarbamate related to protein molecular rearrangements.
complex (FeDETC) encapsulated in a latex rubber matrix. The Nitric Oxide I513 - THERMAL AND MECHANICAL PROPERTIES OF Carlos José Constantino (FCT/Unesp), Rosângela Conrrado (SENAI), Elton Aparecido Reis (FCT/Unesp) and Aldo Eloizo Job (FCT/Unesp) The leather is a material used in the production of several products due Poster (NO) radical is trapped in FeDETC, which allows its detection by electron
paramagnetic resonance (EPR). The latex was used as solid matrix due to excellent biocompatibility, natural angiogenesis properties; thus a promising biomaterial.
to its properties. However, its applications require the treatment process I508 - INTERACTION BETWEEN CHITOSAN AND CELL (cure) using chrome 3. In the curtailment process are obtained a residue denominated leather powder that is one of the main environmental problems. This work has as main objective to prepare composites obtained through the Maria Elisabete Darbello Zaniquelli (USP - FFCLRP), Thatyane Morimoto mixture of natural rubber (NR) with leather powder (NR/Leather), seeking Nobre (USP - FFCLRP), David Sotero Dos Santos Jr. (University of technological applications such as thermal isolation. The composites were Windsor), Luciano Caseli (USP - IFSC), Adriana Pavinatto (USP - IFSC), obtained by compression at 60ºC, 120ºC and 180ºC in different proportions.
Felippe José Pavinatto (USP - IFSC) and Osvaldo Novais De Oliveira Jr. The interaction of chitosan with DPPC and DPPG lipids was proved using I514 - OPTICAL CHARACTERIZATION OF GFP/OC1OC6- Langmuir monolayers as a cell membrane model. Chitosan adsorbs at lipid monolayers, causing expansion of the monomolecular film and suppression Debora T Balogh (IFSC/USP), Osvaldo N Oliveira Jr. (IFSC/USP), José of a plateau representing a phase transition. Regardless the initial lipid A Giacometti (FCT/UNESP), Marli L Moraes (IFSC/USP) and Clarissa A packing, when chitosan is introduced in subphase, surface pressure increases immediately. Dynamic surface elasticity shows that chitosan adsorption at the lipid monolayer changes the surface elasticity, inferring that chitosan The photoluminescence of a conjugated polymer and fluorescent protein has a remarkably effect at lipid monolayer structural characteristics. has been studied for films fabricated using casting. For these films there is red shift in the spectra of the films in comparison to the pure OC1OC6-PPV from ~590 nm to ~650 nm. This shift shows that it’s possible to tune the photoluminescence spectra of the luminescent polymer using fluorescent Luiz Henrique Garcia-Amoedo (Universidade de São Paulo), Terezinha Andreoli Pinto (Universidade de São Paulo), Luís da Silva Zambom (Fatec-SP), Ana Paula Mousinho (Universidade de São Paulo), Ronaldo Domingues Mansano (Universidade de São Paulo), Marcos Massi (ITA) and Ronaldo Ruas (Universidade de São Paulo) Valtencir Zucolotto (USP), Marli L. Moraes (USP), Vananelia P.N. Geraldo In this work, reactive magnetron sputtering was used to deposit W-DLC films on hard metallic implants for orthopedics applications. The W-DLC thin In this study we immobilized liposomes incorporating an anti-inflammatory films obtained in the processes have very low roughness, high resistance to agent (ibuprofen) into layer-by-layer (LbL) films. In vitro experiments were chemical attack and show a high biocompatibility when compared with other performed to determine the kinetics of the release using fluorescence I511 - IN SITU BIODEGRADATION OF POLYURETHANE SPECTROSCOPY OF POLYELECTROLYTE LAYER-BY- Paulo Barbeitas Miranda (IFSC/USP) and Heurison de Sousa e Silva Eric Cloutet (Un. BDX1), Henri Cramail (Un. BDX1), Valdir Soldi (UFSC), Elenara Lemos Senna (UFSC), Betina Giehl Zanetti Ramos (UFSC) and Redouane Borsali (Un. BDX1) LBL films produced by electrostatic self-assembly of polyelectrolytes are finding many useful applications as biosensors and in optoelectronics. Nanoparticles biodegradation studies are very important for biomedical and For optimizing their applications, we use Sum-Frequency Vibrational pharmaceutical applications. Laser light scattering (LLS) has provided an Spectroscopy to investigate at the molecular level how various adsorption accurate and efficient method to study polymer nanoparticles biodegradation parameters affect the polymeric film structure. Our preliminary results on in terms of the changes of the scattering intensity and the hydrodynamic size PAH/PSS LBL films shows that adsorbed films in contact with the solution distribution of polymer nanoparticles. Using polyurethane (PU) nanoparticles (in situ) are quite disordered but after the drying step (ex situ) some films as a typical example, we have shown that its biodegradation was time become inhomogeneous and show some orientational order for the polymer dependent. The decrease of the Rayleigh ratio and the hydrodynamic radius were the evidences of polyurethane nanoparticles degradation.
I517 - MECHANICAL PROPERTIES OF THE ZRO2 AND Claudinei dos Santos (DEMAR-EEL-USP), Juliana Kelmy Macário de Faria Daguano (DEMAR-EEL-USP), Luis Henrique Perdão Teixeira (DEMAR- EEL-USP), Manuela Harue Koizumi (DEMAR-EEL-USP), Renato Chaves Eric Cloutet (Un. BDX1), Henri Cramail (Un. BDX1), Valdir Soldi (UFSC), Souza (DEMAR-EEL-USP) and Kurt Strecker (DEM-UFSJ) Elenara Lemos Senna (UFSC), Betina Giehl Zanetti Ramos (UFSC) and ZrO and Al O were studied, as material for infrastructure applications in dental prosthesis. Powder batches had been prepared, beyond samples of The miniemulsion process was used to synthesize polyurethane (PU) ZrO and pure Al O , a composed mixture of 80% of ZrO2 and 20% of Al O . nanocapsules in aqueous medium with size ranging from 200 to 300 nm. Compacts were sintered at 16000C, with a isothermal holding time of 120 The polyaddition between a natural polyol and isophorone diisocyanate minutes. The mechanical properties of hardness, fracture toughness had (IPDI) used as monomers was monitored at 60°C, in the absence of catalyst been gotten by the method of Vickers indentation method. Furthermore, and was followed by infrared spectroscopy (FT-IR) in the attenuated total fracture resistance was gotten by the four-bending strength tests. To leave reflection (ATR) mode. The morphology of nanocapsules was investigated of these results, reliability analysis of samples were carried through Weibull by Transmission Electronic Microscopy (TEM) and the particles showed I518 - IMMOBILIZATION OF LIPID VESICLES IN MINIEMULSION POLYMERIZATION OF POLYURETHANE Valtencir Zucolotto (IFSC), Mauricio Baptista (IQ), Marli Leite Moraes Henri Cramail (Un. BDX1), Eric Cloutet (Un. BDX1), Valdir Soldi (UFSC), (IFSC) and Osvaldo Novais Oliveira Jr. (IFSC) Elenara Lemos Senna (UFSC), Betina Giehl Zanetti Ramos (UFSC) and Liposomes were immobilized in nanostructured films using the layer-by-layer electrostatic self assembly technique in conjunction with poly(amidoamine) The influence of surfactant type and concentration on particle size and (PAMAM) dendrimers. The capability of the nanocomposites to bind biological formulation yield of the polyurethane (PU) nanocapsules synthesized by species such as Bovine Serum Albumin (BSA) was investigated via Surface miniemulsion polymerization, was investigated. SDS, Tween 80 and Pluronic Plasmon Resonance (SPR) and vibrational spectroscopies.
F68 were employed as a surfactant in concentration ranging from 5 to 20% (vs monomer concentration). The particle diameter slowly decreases from 292 nm to 261 nm as the amount of tween 80 surfactant increases from 5 to 20 wt%. The yield of the formulation was increased using higher surfactant I521 - NANOSTRUCTURED LAYER-BY-LAYER FILMS OF TETRASULPHONATED METALOPHTHALOCYANINES FOR Valtencir Zucolotto (IFSC), Luiz Henrique S. Gasparotto (DQ), José Roberto Siqueira Jr. (IFSC) and Osvaldo Novais Oliveira Jr. (IFSC) DIAMOND-LIKE CARBON FILMS IN BIOPROSTHETIC We report the fabrication and characterization of nanostructured Layer- by-Layer (LbL) films using chitosan (Ch), as a cationic polyelectrolyte, Terezinha Andreoli Pinto (USP), Ronaldo Domingues Mansano (USP), and tetrasulphonated metalophthalocyanines of nickel (NiTsPc), copper Luiz Henrique Garcia-Amoedo (USP) and Ana Paula Mousinho (USP) (CuTsPc) and iron (FeTsPc), as anionic polyelectrolytes. Electrodes with LbL films were used for detecting dopamine (DA) and ascorbic acid (AA), in the Absract: In this work, we have deposited nano-structured Diamond-like concentration range from 5 x 10-6 to 1.5 x 10-4 mol L-1. The limit of detection Carbon films over collagen fibers to provide a sterile hindrance, where crystal of DA and AA was obtained using the calibration equation for each electrode, growth might occur. The results are promising, it is necessary to evaluate a series of chemical, physical, biological parameters, considering many commercial and therapeutic applications. It was possible see the efficiency of the DLC films to decrease the calcification process in bioprosthetic hearth valves.
MICROPARTICLES PREPARED BY EMULSION/SOLVENT I526 - BIODEGRADABLE AMPHIPHILIC NANOSPHERES Alfredo Tibúrcio Nunes Pires (UFSC), Elenara M. T. Lemos-Senna (UFSC) Shirley Possidonio (USP), Walker Soares Drumond (USP) Microparticles containing piroxicam (PXC), an anti-inflammatory drug, This study describes the preparation and characterization of nanocontainers were prepared using poly (hydroxybutyrate) [PHB], by emulsion/solvent for drug delivery systems, from a series of amphiphilic triblock copolymers, evaporation technique. The microparticles were evaluated in terms of poly(l,l-lactide)-block-poly(ethylene glycol)-block-poly(l,l-lactide) (PLA-PEG- morphology, encapsulation efficiency, thermal analysis and in vitro drug PLA). The nanocontainer formation was characterized by 1H-NMR and UV release. The results obtained indicate that the PHB/PXC microparticles can be considered as a promising system for drug delivery applications.
Vladimir Sevastianov (RAS), Alexey Vasiliev (Kurchatov Institute), Leandro Lorenzelli (ITC-Irst) and Vittorio Guarnieri (ITC-Irst) A new microhotplate for semiconductor and thermocatalytic gas sensors Poster I527 - ULTRA-THIN FILMS OF NATURAL RUBBER LATEX
Antonio Riul Jr (UNESP), Celina Massumi Miyasaki (UNESP), Mariselma Ferreira (USP), Aldo Eloizo Job (UNESP) and Carlos José Leopoldo A fundamental study on the fabrication and characterization of ultra-thin films of natural rubber latex (NRL) is presented. Dip-coated films were evaluated based on doped nano-particle oxides has been reported. The aim of this according to the latex concentration, dipping time and pH of the solutions. work is to discriminate different compounds in a gas mixture. In order to A good linear growth of the films could be achieved in all pH values tested. guarantee the sensor sensitivity, stability and low-cost a combined approach Therefore, over a certain limit of NRL diluted in ultrapure water a disordered involving both micro- and nano technologies has been proposed. Concerning growth was observed. The latex adhesion onto quartz substrates was also the sensing layers, we have chosen two different nano-powder materials: checked throughout successive washings in distilled water. Ongoing studies Yttria stabilized Zirconia (YSZ) as a support of thermocatalytic catalyst and are being undertaken combining NRL with chitosan and natural gums Tin dioxide. The application field mainly concerns the building automation systems.
I535 - DETECTION OF PHOSPHATE IONS USING Laércio Gomes (IPEN), Ronaldo Domingues Mansano (EPUSP), Lilia Osvaldo N. Oliveira Jr (IFSC), Ubirajara P. Rodrigues Filho (IQSC), Marli Coronato Courrol (FATEC-SP), Flávia Rodrigues de Oliveira Silva (EPUSP) Moraes (IQSC) and Marystela Ferreira (UNESP) Phytase has been immobilized in Layer-by-Layer (LbL) films, adsorbed Complexation of Eu(III)-tetracycline was studied by luminescence. It alternately with poly(allylamine) hydrochloride (PAH) layers, onto an was proposed to use the lanthanide complex as analytical forms for the ITO substrate modified with Prussian Blue (PB). This film was used to determination of hydrogen peroxide and urea hydrogen peroxide in aqueous- determine phytic acid using amperometric measurements. The results solution. The absorption, fluorescence, and infrared spectra were obtained showed the presence of phosphate ions to confirm detection of phytic acid. for the characterization of the better compositions of the complexes.
I530 - A SIMPLE METHOD TO SYNTHESIZE SILVER Ronaldo Domingues Mansano (EPUSP), Flávia Rodrigues de Oliveira [RUCL2(VPY)4]-4- VINYLPYRIDINE -DIVINYLBENZENE Silva (EPUSP), Lilia Coronato Courrol (FATEC-SP) and Nilson Dias Vieira WITH SILVER NANOPARTICLES FOR ANTIBACTERIAL Eric Nunes Oliveira (UFSC), Karen Segala (UFSC), Rosilene Linhares Dutra (UFSC) and César Vitório Franco (UFSC) In this work photoinduced method for obtaining silver nanoparticles was investigated using UV LED excitation, Xenon lamp and Sodium lamp. The The synthesis and characterization of a new terpolymer with nanosilver is silver colloidal solutions were prepared using autopolymerization resin and described. The terpolymer was synthesized using the monomers in equimolar proportions. The system was maintained under reflux, with vigorous mechanical stirring, under inert atmosphere, at constant temperature. The nanometric dimension of silver nanoparticles was monitored by UV–visible and TEM and was found in the range of 9 to 16 nm. The terpolymer was I531 - LANGMUIR FILMS OF LIGNINS EXTRACTED characterized by SEM, TGA, DSC and GPC. The antimicrobial action was evaluated using S. aureus and E. coli were confirmed through the presence ORGANOSOLV-CO2 SUPERCRITICAL PULPING PROCESS of an inhibitory halo in the culture media. Antonio Aprigio Silva Curvelo (USP/IFSC), Daniel Pasquini (USP/IFSC), Patrícia Alexandra Antunes (Unesp/FCT), Bruna Arlotti Stracçalano (Unesp/FCT), Alvaro Augusto Pereira (Unesp/FCT), Gislaine Felippe Martins (Unesp/FCT), Antonio Riul Junior (Unesp/FCT) and Carlos José I540 - CURCUMIN IMPREGNATION ONTO CHITOSAN Inês M. C. Brighente (UFSC), Tereza C. R. de Souza (UFSC), Alexandre L. Lignins extracted from sugar cane bagasse using different alcohols in the Parize (UFSC), Valfredo T. de Fávere (UFSC) and Mauro C. M. Laranjeira organosolv-CO2 supercritical pulping process have been applied in the fabrication of Langmuir films. These films were characterized by surface pressure-mean molecular area (pi-A) isotherms to exploit information at the This study investigates curcumin impregnation onto chitosan microspheres molecular level, which is a very important issue since even the molecular in order to obtain samples with high nutraceutical potential. The material structure of lignins is still opened in the literature. loaded with dye was characterizated by DSC and SEM. Release studies of the dye were performed under different pH conditions, being observed a protection to the active material against adverse conditions.
molecules playing an important role in protein-phospholipid interactions.
ALLOYS OF POROUS TITANIUM RECOVERED WITH CVD I546 - INTERACTION BETWEEN NATURAL RUBBER and Teófilo Miguel de Souza (FEG - UNESP) AND POLYANILINE INVESTIGATED BY ELECTRIC The CVD diamond was utilized for recover dental implant with alloys of Antônio Riul Junior (UNESP), Rebeca Delatore Simões (UNESP), Adriana porous titanium, with the objective of reduce the rejection upon implanting. Segato Cavalheri (UNESP), Carlos Leopoldo Constantino (UNESP) The dental implants were clear with enzymatic detergent. The analyses used SEM and EDX. The film morphology showed not homogeneous, what is important for the bone one-integration.
Conductive rubbery blends, produced using polyaniline (PANI) depend on several factors such as the type and size, as well as conditions as temperature and pressure. In this work blends of Natural Rubber and PANI were prepared and characterized by electrical properties and Raman scattering to check the miscibility of the materials in each sample, which may be applied as pressure sensor. The mixture was pressed at 180ºC and films with thickness in the order of 0.5 mm were obtained with six different Mauro César Marghetti Laranjeira (UFSC), Nelson Jhoe Batistela (UFSC), proportions (wt.%) which revealed an chemical interaction between both Maurício Rigoni (UFSC), Valfredo Tadeu Fávere (UFSC) The objective of this study is the synthesis and characterization of the iron oxide magnetic particles coated with chitosan by spray-drying method and I547 - MEASURES OF ELECTRIC CONDUCTIVITY preparation of ferrofluids for biomedical applications. When the ferrofluid samples were submitted to an alternated magnetic field, it was observed OF COTTON FABRIC TREATED WITH POLYANILINE variation in the temperature of 7ºC in 40 minutes. Considering this variation Gabriel Galbes Battistone (FCT - UNESP), Geusilange in the temperature is significant given that body temperature is 37ºC and that Santana Silva (FCT - UNESP) and Aldo Eloizo Job (FCT - UNESP) cancerous cells perish around of 42-46ºC. The materials were characterized by scanning electron microscopy and energy dispersive X-ray. Vegetable fibers have been used as reinforcements for polymeric matrix. The use of these fibers is quite advantageous, due to their properties of mechanical resistance, low cost and lightness. Blankets of cotton fabric were treated with conductive polyaniline using the in situ polymerization method varying the treatment time. The samples were characterized BIODEGRADABLE DRUG DELIVERY SYSTEMS (DDS) through measures of electric conductivity on the applied pressure and with accompaniment of the variation of the thickness. The blankets present Maria Ines Re (IPT-USP), Andreza Ribeiro Simioni (FFCLRP-USP), conductivity in the order of 10-3 S.cm-1 within the interval from 0.05 to 0.5 The potential use of PHBHV/PCL was investigated in this work to prepare drug-loaded microspheres by emulsification-solvent evaporation method. NzPC, a photosensitizer dye, whose application in photodynamic therapy (PDT) against cancer has been investigated, was incorporated into the PHBHV/PCL microspheres as a model drug. The biodegradation studies Maria Elisabete Darbello Zaniquelli (FFCLRP-USP), Rosa dos Prazeres were done to investigate the effects of the incorporation of the NzPC and Melo Furriel (FFCLRP-USP), Francisco de Assis Leone (FFCLRP-USP), also to determine the profile release.The results showed that PHBHV/PCL César Vanderlei Nascimento (FFCLRP-USP) blend may present good biodegradable properties to control the release of Phospholipid compositions can tailor the biomembrane fluidity, which is important for the enzymatic activity as well as other kinds of bioprocesses. Langmuir monolayers (LM) and Langmuir-Blodgett (LB) techniques provide the immobilization of enzymes on phospholipid films, with the advantage that FACTORS (FGFS) WITH PHOSPHOLIPID SURFACE allow fluidity modulations by changes the composition and packing of the matrix. In this work, we have studied the influence of iota carrageenan, a Osvaldo Novais Oliveira Jr. (USP), Wilker Caetano (UNESP), Mariselma sulfated polysaccharide, on sodium dihexadecylphosphate (DHP) LM and LB Ferreira (USP) and Marystela Ferreira (UNESP) films and subsequent immobilization of a soluble alkaline phosphatase.
In this work, Pi-A isotherms and hysteresis studies have been used to investigate the dynamic adsorption/desorption of the bFGF (basic fibroblast growth factor) in phospholipid Langmuir monolayers under a subphase containing protein. The results evidenced that the protein, even at nanomolar concentrations (10-9 up to 10-7 M), alters the monolayer properties differently for zwitterionic and anionic phospholipids, while the charge in the 127 Hidetake Imasato (IQSC-USP), Pedro Augusto de Paula Nascente (UFSCar), Paulo Sérgio de Paula Herrmann Jr (Embrapa), Fábio de Lima Leite (IFSC- USP), Julio Carlos Ribelatto (IQSC-USP), Juliana Salvador Andresa (IQSC- I550 - REAL-TIME ATOMIC FORCE MICROSCOPY IMAGING OF DIBUCAINE-INDUCED CONFORMATIONAL Eneida de Paula (IB/UNICAMP), Gabriela Simone Lorite (IFGW/ UNICAMP) and Mônica Alonso Cotta (IFGW/UNICAMP) We have investigated the effects of the interaction between the local USP) and Ubirajara Pereira Rodrigues-Filho (IQSC-USP) anesthetic dibucaine and supported egg phosphatidylcholine bilayers in aqueous environment by atomic force microscopy. With increasing dibucaine This work describes a comparative study of the morphologies of the hemoglobin concentration in the liquid cell during imaging, a progressive decrease in the monomer and hemin films on SiO2/Si wafer surface previously modified with size of the lipid domains was observed up to disruption of the bilayer.
3-Imidazolylpropyltrimethoxysilane (3-IPTS) using Atomic Force Microscopy (AFM) and Scanning Electronic Microscopy (SEM). The hemoglobin monomer and hemin (iron protoporphyrin IX, FePPIX) adsorptions were confirmed due to the presence of Fe2p photoemission line in the long-scan XPS. The root- I551 - BIOCOMPATIBILITY OF MULTI-WALLED CARBON mean-square roughness (Rrms), measured by AM-AFM of monomer was Anderson Oliveira Lobo (INPE/ITA), Erica Freire Antunes (INPE), Mariana Bernardes Palma (INPE/UNIVAP), Cristina Pacheco Soares (UNIVAP), Vladimir Jesus Trava-Airoldi (INPE) CARBONATE FILMS FORMED ON A POLYMERIC MATRIX Maria Elisabete Darbello Zaniquelli (FFCLRP-USP), Fábio G. Doro This work-studies cell adhesion and cell viability of multi-walled carbon (FFCLRP-USP), Elia Tfouni (FFCLRP-USP) and Ana Paula Ramos (FFCLRP- nanotubes (MWCNTs) films grown on titanium and silicon surfaces. Microwave plasma was used to produce quite pure carbon nanotubes. Fibroblast L929 mouse cells were used for biocompatibility tests, such as Organic-inorganic thin films were deposited on aluminum substrates MTT assay and cellular adhesion. The results show very high cell viability employing the layer-by-layer technique combined with a chemical reaction and many layers of cells on the surface formed by the nanotube tips. using the reactant from vapor phase. Poly(acrylic acid) and chitosan were used as negatively and positively charged polyelectrolytes, respectively, forming an organic matrix in which calcium carbonate was deposited. The coatings are Poster Session PI2 - Thursday October 12
dense, homogeneous and results in hydrophilic surfaces, characterized by optical microscopy, scanning electron microscopy, X-ray diffraction, ATR-FTIR and dynamic contact angle measurements.
I557 - EFFECT OF FILM THICKNESS AND ELECTRODE Adriana Raffin Pohlmann (UFRGS), Sílvia Stanisçuaski Guterres (UFRGS) GEOMETRY ON THE PERFORMANCE OF TASTE SENSORS MADE OF NANOSTRUCTURED CONDUCTING POLYMER Sodium alendronate (SA) is used in the treatment of osteoporosis and has Fernando Joseppeti Fonseca (Escola Politecnica da USP), Leonardo also been associated with gastrointestinal side effects. Regarding the oral Giordano Paterno (Escola Politecnica da USP), Nadja Karolina Leonel administration, microparticles (MP) were widely proposed to carry drugs in Wiziack (Escola Politecnica da USP) and Luiz Henrique Capparelli Mattoso order to increase their efficacy and/or decrease their toxicity. The aim of this work was to prepare and to characterize MP containing SA using Eudragit S100 by spray-drying. The yield was 62 %, the encapsulation efficiency was In this contribution, the effect of film thickness and electrode geometry on 78 % and the mean particle size was 21 micrometers presenting Span of 1.9. the performance of taste sensors made of nanostructured films of conducting SEM analysis showed that MP are collapsed and presented smooth surface.
polymer poly(o-ethoxyaniline)-POEA and sulfonated polystyrene-PSS was investigated. The thickness of POEA/PSS films and the interdigitated microelectrodes-IME geometry presented significant influence on the sensors response. I553 - SYNTHESIS AND CHARACTERIZATION OF NEW Karoline Bastos Mundstock (UFSC) and Mauro Cesar M Laranjeira (UFSC) I558 - DSC STUDY OF DRUG-LOADED PHBHV/PCL The objective of this paper is the synthesis and characterization of a new cel ulose fiber reinforced cement composites containing calcium phosphate(ACP/CPP), Adriana Raffin Pohlmann (UFRGS), Sílvia Stanisçuaski Guterres (UFRGS), chitosan (QTS) and polyvinyl alcohol (PVA), which might be used as cement Maria Inês Ré (IPT-SP) and Maria Ismenia Lionzo (UnC) for bone repair. The samples were characterized by infrared spectroscopy, X-Ray Dispersive Energy Analysis and, X-ray diffraction analysis. The addition DSC analyses were used to investigate the association mechanism of the of cel ulose fiber improved mechanical resistance to compression from 80% hydrophobic drug models dexamethasone and dexamethasone acetate in the cylinder containing C-ACP -QTS, 200% in the cylinder containing C-ACP loaded in microparticles prepared with blends of poly(hydroxybutyrate- - QTS/PVA blend and 80% for cylinder containing CPP – QTS.
cohydroxyvalerate) [P(HBHV)] and poly(-caprolactone) (PCL). The entrapped drug influenced the melting point and the glass transition temperature of P(HBHV) denoting that the drug is associated with the applications. The first is a soft method for the dispersion of CNTs in water by using ammonium hydroxide. It results in the non-covalent attachment of NH3 groups to the CNT walls. The second method results in to the non-covalent attachment of l-alanine, which will enable the sub-sequent binding of peptides. In both methods, high solubility of CNTs in water is achieved.
Volney Mattos Oliveira (UNICAMP), Itamar Ferreira (UNICAMP) and I563 - EVALUATION OF OXIDATE POLYETHYLENE Maria Angelica Alves Mattos de Olivera (UNICAMP) MORPHOLOGICAL AND BIODEGRADATION PROPERTIES The purpose of this paper is to obtain a process conditions for refining the microstructure of the Ti-35Nb-5Ta-7Zr alloy from a casting condition. The Cristina das Graças Fassina Guedes (USF), Maria Regina Calil (USF), start materials were ingotes fabricated in an arc voltaic furnace, in vacuum Flávia Gaboardi (USF) and Derval dos Santos Rosa (USF) with addition of inert gas. The Ti alloy presents advantages in relation to the other Ti alloys because its low modulus of elasticity and all the used Blends of PHB and LDPE with and without 5 wt% of OPW were developed elements are biocompatible. By using hot rolling, at 1230o C, and swaging and characterized. The mechanical results showed that the addition of OPW (radial forging), at 700ºC, it was possible to obtain a good homogeneity and increased the tensile strength and Young´s modulus but decreased the elongation at break of the. DSC showed that the addition of OPW caused a small decrease in Tg. The incorporation of OPW increased the mean values for the MFI. OPW altered the morphology of LDPE by decreasing the phase separation. In biodegradation experiments, the loss of mass by the was greater in the presence than in the absence of OPW.
Luís Antônio da Silva (UFVJM), Alexandre Rossi (UFVJM), Érmeson L. de O. Teixeira (UFVJM), Luís Fernando de Santos Faria (UFVJM) and Valéria I564 - EPR AND XRF STUDY OF THE ELIMINATION KINETICS IN BLOOD AND DISTRIBUTION IN LIVER The corrosion resistance of CuZnAl odontological alloys in presence of carbonate buffer in presence of 0, 10 and 50 mM of chloride was evaluated. Angela Maria Falleiros (UEL), Ernanni Damião Vieira (IFSC-USP), The polarization curves (in 0 and 10 mM of Cl-) presented an wide potential Antonio da Costa Filho (IFSC-USP), Edson Amaro Junior (INRAD-USP), region of formation of a passive film on the alloy surface. At ~ +1.0V it Giancarlo Esposito Brito (IF-USP), Lionel Gamarra Contreras (IF-USP), was observed the transpassivation potential. The analysis of the polarization Sylvia Mendes Carneiro (Ins. Butanta), Vera Lucia Salvador (IPEN), Doris curve in 50 mM of Cl- shows that the potential region is comparatively Maribel Escriba (IF-USP) and Walter Maigon Pontuschka (IF-USP) narrower. Passivation is the main process for the copper-based in carbonate buffer (0 and 10 mM of chloride). The presence of 50 mM of Cl- difficults it.
The elimination kinetics in blood and biodistribution in liver of ferrofluids administred are studied in rats using two alternative techniques: EPR and X-ray fluorescence (XRF). We found a 12 min half-life persistence of iron oxide nanoparticles in the blood stream, and maximum liver concentration was attained circa 95 min after administration. Carla Eiras (UFPI), Valtencir Zucolotto (IFSC/USP), Débora Gonçalves (IFSC/USP), Maurício Foschini (IFSC/USP), Ana Cristina F. Brito (UFC) I565 - THE EFFECT OF CORROSION ON NEODYMIUM IRON BORON MAGNETS USED IN THE ORTHODONTIA In this work, we take advantage of the polyelectrolyte character of Brazilian Sisenando Itabaiana Sobrinho (UEMG), Sebastiana Bragança Lana native gum to fabricate electrically conductive, nanostructured films. (UEMG) and Nelcy Della Santina Mohallem (UFMG) Anacardium occidentale L. (cashew gum) was assembled in conjunction with polyaniline (PANI), using the layer-by-layer (LbL) technique. The adsorption This investigation analyzed the effects of the corrosion in the structural and process of the PANI/gum bilayers was monitored via quartz crystal magnetic properties of NdFeB magnets used in orthodontic desimpaction. NdFeB magnets were preliminarily analyzed by electron microprobe, scanning electron microscopy and small angle X-ray diffraction. For the corrosion tests, the samples were immersed in a solution of lactic acid 1% and kept at 37°C for 70 days. The magnets had been removed from the acid I562 - FUNCTIONALIZATION OF CARBON NANOTUBES solution in intervals of 7 days for analyses. The samples were structurally and morphologically characterized, and submitted to magnetic tests.
Rodrigo G Lacerda (UFMG), Luiz O Ladeira (UFMG), Marcos A Pimenta (UFMG), Andre S Ferlauto (UFMG), Ariete Righi (UFMG), Sergio Oliveira (UFMG), Hallen D Rezende (UFMG) and Edelma E Silva (UFMG) I566 - INFLUENCE OF THE FABRICATION PARAMETERS In recent years, carbon nanotubes (CNTs) have been recognized as potentially important biomaterials. In this work, it is demonstrated two methods of MORPHOLOGY OF PANI/PSS SELF-ASSEMBLED FILMS CNT functionalization, which represent the initial steps towards biological John Paul Hempel Lima (EPUSP), Leonardo Giordano Paterno (EPUSP), Dengue is an important disease caused by an arbovirus worldwide, especially in the tropical regions. We used AFM to investigate the molecular interaction between dengue virus and Aedes aegypti cells. For this, silicon tips functionalized with this virus and the tick-borne cells was used as a substrate for AFM analysis.
PANI/PSS self-assembled films were fabricated to verify the influence of fabrication parameters (such as concentration and pH of the polymeric soluions; washing and drying steps) on their morphology and electrical characteristics. The effeciviness of the assembly methods (manual versus mechanical) were also studied, thereto, a homemade device was employed on the assembly procedure and the films assembled were compared with HYDROXYAPATITE BASED CERAMICS WITH DIFFERENT Ana Helena Almeida Bressiani (IPEN), Márcia Martins Marques (FOUSP), Juliana Marchi (IPEN), José Carlos Bressiani (IPEN) and Carina Sinclér Flávia Gaboardi (USF), Derval dos Santos Rosa (USF), Maíra de Lourdes The aim of this study is to investigate the influence of different ceramics (Al O and HAp), submitted to different superficial treatments (as sintered, The mechanical properties of PCL/PVC blends and their biodegradation in rectified and polished) on the biocompatibility. Sintering was accomplished simulated soil were evaluated. The blends showed a lower tensile strength, and density was evaluated. The roughness was determined and after elongation at break and elasticity modulus than pure PCL. The biodegradation sterilization (Co60 Gammacell), 1 x 105 fibroblasts were plated. After 1, rate increased with increasing PCL content of the blends, and blends with 2 and 3 days, the samples were prepared for SEM. It was concluded that the plasticizer DOA had the greatest biodegradability. the rectification influences negatively on the biocompatibility of the HAp, whereas the rectification and polishing improve the biocompatibility of the Al O I569 - INTERACTION BETWEEN ANTIMICROBIAL I575 - SYNTHESIS AND CHARACTERIZATION OF CAP/ Roseane Rigo (IFSC - USP), Rosângela Itri (IF - USP), Diana Müller (UNL), Georgina Tonarelli (UNL), José Luiz de Souza Lopes (IFSC - USP) Luis Guilherme Dias Heneine (FUNED), Maria Helena - Santos (UFVJM) In the search of understanding the mechanism of action of some members Calcium phosphate/collagen composites are promising biomaterials however from bacteriocin group (Plantaricin 149, Pediocin A and modified forms), the have a rapid bioresorbability. The addition of Zn+² in these materials can interaction of these peptides with biomimetic systems composed of different be stimulated the osteoblast proliferation and bone formation. Composites phospholipids and detergents have been analyzed by CD, fluorescence, SPR, were developed mixing calcium phosphates doped and undoped with zinc calorimetric and in vitro studies, showing a high conformational change due and pure collagen type I. The characterized samples presented morphologic to the induction of a helicoidal content on the peptide’s secondary structure and structural characteristics similar to their constituents and the biological that can occur by electrostatic interactions between the positively charged evaluation in vitro showed cytocompatibility. Probably, the doped Zn+² residues of Lys of the peptides and the anionic head of phospholipids.
biocomposites can significantly influence the rate of bone reconstruction.
I571 - STUDY OF SUBSTANCES DELIVERY FROM LATEX I577 - MODELING OF AGGREGATION PROCESSES IN FILM USING SPECTROPHOTOMETRY LOWRY METHOD AND UV APPLIED TO BONE GUIDED REGENERATION Helinando Pequeno de Oliveira (UNIVASF), Felipe Nascimento de Souza Cibele Ereno (USC), Carlos F. O. Graeff (FC - UNESP), Rondinelli Leão (UNIVASF), Thiago Fernandes dos Santos (UNIVASF) and Celso Donizetti Herculano (FFCLRP - USP), Cecília Pereira Silva (FFCLRP - USP), Sérgio A. C. Guimarães (USC) and Angela M. O. Kinoshita (USC) Electrical impedance spectroscopy has been established as a tool of In this work, we characterized the release of substances from latex films by fundamental importance for the study of molecular organization in aqueous Spectrophotometry with the purpose of future use as a barrier membrane and solid state systems in the presence of an external field. In this work and a protein delivery system simultaneously for dental and orthopedic we examine the process of micelle formation in an aqueous environment by use of a computational simulation based on first-neighbors charge transport by percolation through a 2-D mesh of surfactant and water molecules. By varying the concentration of the components we follow the evolution of the resistance of the circuit. I573 - MODIFIED AFM PROBES FOR HIGH RESOLUTION IMAGES AND STUDY OF MOLECULAR INTERACTIONS BETWEEN DENGUE VIRUS AND AEDES AEGYPTI CELLS Erivelton F Costa (UFC), Luciana M Rebelo (UFC), Marcelo R Oliveira I578 - STUDY OF CHITOSAN EFFECTS IN LIPOSOMES (UFC), Maria Isabel F Guedes (UECE) and Jose A K Freire (UFC) Nádya P. da Silveira (UFRGS), Adriana R. Pohlmann (UFRGS), Marc Schmutz (Institut Charles Sadron), André Schroder (Institut Charles Sadron), Carlos M. Marques (Institut Charles Sadron) A structural study on nanoliposomes under chitosan influence was Ana Luisa Bravo de La Corsa (UNICAMP), Priscyla Daniely Marcato performed. Samples with increasing chitosan concentrations were analysed (UNICAMP) and Nelson Durán (UNICAMP/UMC) by scattering techniques. An increase in size and polydispersity upon the addition of chitosan was observed. Zeta potential was higher for samples The aim of this work was to study the enzyme encapsulation by double with chitosan, whereas lamellar dimension was reduced. SAXS shows emulsion method with and without salt in the external phase. The protein the presence of higher contents of multilamellae by increasing chitosan. model was peroxidase. Nanoparticles with 230 nm of size were obtained Electronic microscopy corroborates some results. Results with giant with an encapsulation efficiency of 40%. liposomes and micropipette technique show the lamellar elastic properties of liposomes containing chitosan.
I586 - INFLUENCE OF (V) NIOBIUM PHOSPHATE I579 - ELECTRIC CHARACTERIZATION OF NATURAL André Vitor Chaves Andrade (UEPG), Sandra Regina Masetto Antunes (UEPG), Andressa Fantin (UEPG), Mariza Boscaci Marques (UEPG), PARAMETERS RELATED TO FABRICATION AND AGING Augusto Celso Antunes (UEPG), Lucas Anedino de Souza (UEPG) and Mariselma Ferreira (FFCLRP-USP), Valtencir Zucolotto (IFSC-USP), Natália Destefano (FFCLRP-USP) and Marcelo Mulato (FFCLRP-USP) The influence of niobium phosphate in hydroxyapatite (HAP) and its The natural rubber polymer has been the object of interest for applications interference in the biomaterial behavior were studied in this work. The as biomaterial in view of its excellent bio-compatibility and the natural HAP was prepared through the chemical precipitation method. The samples stimulation of angiogenesis. An initial study of the electric properties of this were MEV and DRX characterized, and the phases quantified by the Rietveld polymer has been developed and it showed that there are some fabrication Method. After characterizations, in-vitro tests were carried out to analyze parameters related to physical mechanisms that influence the final result the material biocompatibility. The X-ray analyses revealed that pure HAP obtained by Complex Impedance Spectroscopy. Electric measurements were presents two phases: HA e beta-TCP, however, by adding niobium phosphate carried through as a function of these parameters, like the thickness of the membranes and the aging of the films.
I580 - FTIR SPECTROSCOPY CHARACTERIZATION OF CHANGES ON MEMBRANE PHASE TRANSITION AND POLY (VINYL ALCOHOL) HYDROGEL WITH DIFFERENT HYDROLYSIS GRADE AND CHEMICALLY CROSSLINKED Maria Inês Bruno Tavares (UFRJ), André Avelino Pasa (UFSC), Miguel Soriano Balparda Caro (UFSC), Maximiliano Luis Munford (UFSC), Vânia Herman Sander Mansur (UFMG), Carolina Sadahira (UFMG) Rodrigues de Lima (UFSC) and Tânia Beatriz Creczynski-Pasa (UFSC) AFM, fluorescence and NMR techniques were used to investigate melatonin- The purpose of this study was to characterize through FTIR spectroscopy induced modifications on membrane phase transition, molecular packing the chemical structure of PVA hydrogels crosslinked with glutaraldehyde and mobility at selected phospholipids bilayer regions. NMR analysis and evaluate the influence of the Hydrolysis grade (GH) on the crosslinking suggests region-specific opposite melatonin effects. Fluorescence results and crystallinity of PVA films. It was found a curve correlating GH and the show a melatonin-induced fluidizing effect on lipid acyl chain. Melatonin absorbance ratio between C-O (1145-1142 cm-1) with C-O-C (1150-1085cm- also seems to retard mica supported-lipid phase transition, as observed on 1)3. Based on the results obtained it was observed an increase of the the AFM images. The strategies handled in this work can contribute to the crystalline phase with higher hydrolysis grade of PVA.
understanding of drug delivery systems and biosensors.
I583 - PHASE TRANSFORMATION IN POLYETHYLENE I588 - IMMOBILIZATION OF RS100A12 PROTEIN IN GLYCOL INDUCED BY ATOMIC FORCE MICROSCOPY Margareth Spangler Andrade (CETEC), Vanessa Carla Mosqueira (UFOP), Ana Paula Ulian Araújo (IFSC-USP), Osvaldo N. Oliveira Junior (IFSC- Elaine Amaral Leite (UFMG) and José Mário Vilela (CETEC) USP), Valtencir Zucolotto (IFSC-USP) and Assuero Faria Garcia (IFSC- This work was performed on an aqueous polyethylene glycol (PEG) solution placed on mica surface. Atomic force microscopy (AFM) images showed that rS100A12 porcine was immobilized by layer-by-layer technique in the PEG chains appeared stretched, coiled or as planar lamellae. Partially poly(amidoamine) generation 4 (PAMAM G4) dendrimer matrices. crystalline spherulites and amorphous agglomerates, with a calotte shape, Immobilization of rS100A12 was performed in the presence of Zn2+ ion and were also observed. When imaging the amorphous agglomerates they can the bilayer adsorption was monitored by fluorescence spectroscopy. Circular change their shape to show the aspect of spherulites. This morphology dichroism (CD) analysis showed that the immobilized rS100A12 has the same change is attributed to a phase transformation from a meta-stable �-helical structure as presented when in solution.
amorphous to a partially crystalline state induced by the forces between AFM tip and sample. I596 - POLYMERIC NANOCAPSULES CONTAINING RETINYL PALMITATE FOR TOPICAL APPLICATION Nelson Durán (IQ/UNICAMP), Sílvia Staniçuaski Guterres (FARMÁCIA/ Poster I589 - STRUCTURAL CHARACTERIZATION OF A MIXED
REVERSED MICELLAR SYSTEM: A SMALL ANGLE X-RAY Rosangela Itri (IFUSP), José Fábio Gomes (IFUSP), Carmem Lúcia Amaral (Unicsul) and Leandro Ramos Barbosa (IFUSP) Retinyl palmitate nanocapsules were prepared for the first time. It was In this work, we made use of small angle x-Ray scattering (SAXS) technique employed the poly(D.L-lactide) biodegradable polymer functionalized with a to get the structural features of mixed reverse micelles (MRM) composed fluorescent probe. The nanoparticles showed low polydispersion index and of AOT and HDEHP. Resersed Micelles (RM) have been extensivelly used as a high active load. The assays in abdominal human skin by Confocal Laser a tool on protein extraction field, because of its ability on keep the protein Scanning Microscopy showed a suitable permeation. These are promising biological activity. Our SAXS results give support to conclude that the MRM results to technological applications, such as anti-aging creams.
preserve their spherical shape but increase in size as the HDEHP ratio increases in the system. It means that the presence of HDEHP induces micellar growth,probably due to the excess of free water in the inner core of the MRM.
I597 - STUDY ON THE CHEMICAL STABILITY OF I591 - DENDRIMERS AS NANO-CONTROLLED RELEASE Luiz Henrique Capparelli Mattoso (Embrapa-CNPDIA), Ronaldo Censi Faria (DQ-UFSCar), Leonardo Giordano Paterno (EPUSP-USP), Humberto Eder Lilia Romero (UNQ), Maria Jose Morilla (UNQ) and Ana Paula Perez Hissashi Takeda (DQ-UFSCar) and Orlando Fatibello Filho (DQ-UFSCar) In this contribution, polyaniline and sulfonated-lignin films are fabricated by Abstract. Dendrimers have particular properties that make them useful the layer-by-layer technique and their chemical stability is evaluated by UV- as nano-controlled release system (nano-CRS) for siRNA. In this work, Vis spectroscopy and cyclic voltammetry simultaneous measurements. The complexes formed by PAMAM dendrimers G4 and anti-GFP siRNA were chemical structure of films reversibly changes during the cyclic voltammetry characterized by electrophoresis retard, ethidium bromide displacement, and no loss of polymeric mass is observed after a complete cycle.
size, cytotoxicity and gene silencing efficiency.
Vânia Rodrigues de Lima (PósGrad. Química UFSC), Germano Marcelo Knobel (IFGW-UNICAMP), Tatiana Midori Martins (IFGW- Heinzelmann (Dep. Física UFSC), André Avelino Pasa (Dep. Física UFSC), UNICAMP), Daniela Zanchet (LNLS) and Paula Silvia Haddad (LNLS) Tiago Opala Vieira (Dep.C. Farmacêuticas UFSC), Maximiliano Luis Munford (Dep.C. Farmacêuticas UFSC) and Tânia Beatriz Creczynski- This work reports the syntheses and properties of iron oxides nanoparticles covered by dimercaptosuccinic acid (DMSA). The XRD measurements of the resulting nanoparticles showed to be stoichiometric Fe3O4 magnetite, an Supported phosphoolipid membranes prepared by vesicle fusion were inverted spinel structure, with crystalline domains in range of 4-10 nm. The characterized in-situ with AFM. The phase transition of lipid layers was systems were also characterized by SAXS and TEM. The results showed that observed varying the temperature. It was possible to obtain AFM images the particles have a narrow size distribution and average sizes similar to in contact mode of lipid membranes at the liquid-crystalline phase. A binary the crystalline domain as shown by XRD. The magnetic measurements on mixture of lipids was also investigated.
all nanoparticles showed that the particles are superparamagnetic at room temperature.
CONDUCTING POLYMER AND BRAZILIAN NATIVE GUMS I595 - STRUCTURAL INVESTIGATION OF THE LATEX Carlos Leopoldo Constantino (Unesp), Igor Lebedenco Kitagawa (Unesp) Carlos José Leopoldo Constantino (UNESP/FCT), Deuber Lincon da Silva In this work we have used some brazilian native gums to fabricate Agostini (UNESP/FCT) and Aldo Eloizo Job (UNESP/FCT) electrically conductive nanostructured films. Brazilian gums were assembled in conjunction with poly(o-methoxyaniline) emeraldine salt (POMA-ES) using Hevea brasiliensis. The latex is a colloidal system of negative particles the layer-by-layer (LbL) technique. The characterization of the films has been dispersed in an aqueous serum. An important application of the natural rubber carried out using UV-vis and Raman spectroscopies.
is being accomplished in the biomedical area making possible angiogenesis induction leading to vascularization improvements and showing excellent biocompatibility and absence of any toxicity or allergic manifestations. Latex centrifuged solution and natural rubber films were characterized through I602 - DENDRIMERS: NANO-CONTROLLED RELEASED the FT-IR technique with goal of identifying the active.
Diana Bacigalupe (UNLP), Cecilia Venturini (UNLP), Maria Jimena Prieto I606 - ANTICANCER DRUGS RELEASE FROM SYSTEMS (UNQ), Maria Jose Morilla (UNQ) and Eder Lilia Romero (UNQ) Abstract: High doses of sulfadiazine (SDZ), used in synergistic combination with pyrimethamine, are mainly responsible for severe side effects and Calcium phosphate cements (CPC), particularly calcium hydroxyapatite (HA), discontinuation of toxoplasmosis treatments. As part of our previous search dicalcium phosphate (DCP) and alfa-tricalcium phosphate (alfa-TCP), have for new strategies conducing to improve the efficacy of treatments by been studied as potential candidates for anticancer drugs delivering. CPC- employing low doses of SDZ, we have determined citotxicity, cell damage, drug cements were previously prepared by solid-state reactions between uptake rate, as well as biodistribution and pharmacokinetics of dendrimeric- mixtures of (i) (NH4)2HPO4, CaCO3 and NH4OH (CFC 16), (ii) TCP, DCP and H3PO4 (CFC 21), and (iii)TCP, Ca(OH)2 , H3PO4 and hydroxy carbonate apatite (CFC 29), and four kinds of anticancer drugs solutions: cisplatin (2,5 mg.mL-1), carboplatin (25 mg.mL-1), etoposideo (20 mg.mL-1 ) and mitoxantrone (2,0 mg.mL-1). The mixtures were put into a mortar to obtain pastes of I603 - MORPHOLOGICAL CHARACTERIZATION AND workable consistency. Next, cement samples were manually molding as INFRARED SPECTRA OF ASTAXANTHIN/CHITOSAN small beads (300 mg), and maintained at physiological temperature and relative humidity of at least 90% during 2 days for drying. Twelve CFC- Roberto B. Derner (UFSC), Renata Avila Ozorio (UFSC), Jefferson Rotta drug cements were obtained: CFC 16-cisplatin, CFC 21-cisplatin, CFC 29- (UFSC) and Pedro Luiz Manique Barreto (UFSC) cisplatin,CFC 16-carboplatin, CFC 21-carboplatin, CFC 29-carboplatin, CFC 16-etoposideo, CFC 21-etoposideo, CFC 29-etoposideo, CFC 16-mitoxantrone, Astaxanthin is a highly unsaturated molecule, that can easily be damaged CFC 21-mitoxantrone and CFC 29-mitoxandrone. Each sample was immersed by heat or light, because of that astaxanthin must to be protect, in order to in 50 mL of physiological serum (0.9 % NaCl aqueous solution) at 37 maintain its chemical structure and consequently its biological properties. Celsius degrees under constant stirring. Aliquots of 5 mL were removed Chitosan has been successfully used for the preparation microspheres and replaced with fresh serum solution at predetermined intervals (0, 15, which are currently used in the biomedical, cosmetic and food industries. 30, 45, 60, 180, 360, 1440 and 2880 minutes). Concentrations of released Microparticule of chitosan with astaxanthin encapsulated was prepared. drugs were measured in a UV-visible spectrophotometer Shimadzu 2401 by The SEM micrographs show a non-homogeneous size and a diameter of 33- reading the absorbencies of cisplatin at 212.6 nm, carboplatin at 214.7 nm, 52 �m, with a rather smooth surface. The FTIR spectra of microparticules etoposideo at 282.5 nm and mitoxantrone at 275 nm. Cements chemical and indicating a formation of an astaxanthin-chitosan complex.
morphologic characteristics (molar reason Ca/P, superficial specific area and degree of porosity) as well as the kind of drug charged can have influenced in the kinetic profiles of release of the investigated systems. The cements charged with cisplatin presented release rates significantly faster, mainly in the first hours of experiment. On the other hand, the kinetic profiles of BIOCOMPATIBLE MAGNETIC FLUIDS BIODISTRIBUTION release of carboplatin indicate extremely slower release rates of the drug under the conditions examined. This suggests that the complex formed when Geraldo José da Silva (UnB), Francisco Augusto tourinho (UnB), Adriana carboplatin molecules are bound to crystals of CFC can be more stable due Pereira Ibaldo (IFSC-USP/UnB) and Jerome Depeyrot (UnB) to a stronger affinity between their functional groups oppositely charged. Similar release profiles were found for the systems CFC-etoposideo and We present an original propose of using differential pulse voltammetry CFC-mitoxantrone according to intermediary release patterns. This behavior to monitor in vitro the biodistribution of the nanoparticles in biomedical can be associated mainly to the molecular complexity of the structures of applications of B-MF through the identification of distinct species containg the both drugs and microstructural characteristics of the cements (particles iron (III) from their respective peak potential and dosage relating the peak superficial area, cement porosity and biodegradable behavior). However, current and the concentration of the species. additional similar studies using others physiological media and collagen-CFC composites are already in progress.
I607 - EVALUATION OF THE INFLUENCE OF THE ABSORPTION PROPERTIES OF CACO3 POLYMORPHS PREPARATION OF THE TITANIUM SURFACE USED Francisco Franciné Maia Júnior (UFC), Ewerton Wagner Santos Caetano FOR COATED IMPLANTATIONS ODONTOLOGIC WITH (UFC), Valder Nogueira Freire (UFC), Subenia Karime Medeiros (UFRN) TITANIUM DEPOSITED FOR THERMAL ASPERSION P Ana Paula Vaz (UFPR), Ramon Cortés Paredes (UFPR) First-principles calculations are performed using the CASTEP code within the density functional theory (DFT) for the CaCO3 polymorphs calcite, aragonite, In this work we search to optimize the process of preparation of the surface and vaterite. The exchange correlation potential is considered in the LDA through double abrasive jet and the covering through the titanium deposition and GGA approximations using ultrasoft Vanderbult-type pseudopotentials in dust on titanium substratum through the process plasma spray the empty together with a plane-wave basis set. For the three polymorphs, we calculate motivation is to increase the porosity and, to control the roughness and in detail the structural parameters, charge transfers, bond populations, band to guarantee the necessary tack with the substratum through the biggest structure, density of states, optical absorption, as well as effective masses superficial energy of adhesion thus allowing one better wetting and tack of the bone cells.The results mainly show that the morphology is significantly improved, for the presence of emptinesses and fragmented titanium particles I612 - CHARACTERIZATION OF SELF ASSEMBLED PHOSPHOLIPID BILAYERS ON DITHIOTHREITOL AND André Avelino Pasa (Dep. Física UFSC), María Elena Vela (INIFTA UNLP), MA Daza Millone (INIFTA UNLP), Vânia Rodrigues de Lima (Dep.C. Farmacêuticas UFSC), Tiago Opala Vieira (Dep.C. Farmacêuticas UFSC), BIOMEDICAL APPLICATIONS: DEVELOPMENT AND Rui Nunes Correia (Universidade de Aveiro), Maria Vaz Fernandes (Universidade de Aveiro), Márcio Celso Fredel (UFSC), Nuno Fraga Almeida (Universidade de Aveiro), Bárbara Leite Ferreira (Universidade Maximiliano Luis Munford (Dep.C. Farmacêuticas UFSC) and Tânia de Aveiro) and Marcelo Peixer Corbellini (UFSC) Beatriz Creczynski-Pasa (Dep.C. Farmacêuticas UFSC) New composites of PMMA-co-EHA were developed and their preliminary This work presents the characterization of self-assembled lipid bilayers mechanical properties were obtained. The composites were based on on dithiothreitol (DTT) and dithioerythritol (DTE) treated gold thin films, commercial two-solution acrylic bone cements used commonly in the evaporated on silicon. The interest on gold surfaces is to preserve this treatment of bone defects. One ceramic glass filler was used and the substrate as an electrode for detection of charge transfer processes for influence of different proportions of this filler was studied. The composites fundamental studies and biosensor applications. The isomers DTT and were obtained by the mixing of liquid and solid components though the DTE were used in a tentative to elucidate the mechanism of the molecules polymerization was reached by the addition of an initiator and an activator. binding on gold substrate. To obtain the lipid bilayers dimyristoylphosphatid The composites were produced successfully and the filler was benefic to the ylcholine (DMPC) was supported on gold by vesicle fusion. The morphology of the structures obtained were analyzed by in-situ atomic force microscopy inside a fluid cell with HEPES buffer (pH = 7.4), under contact mode with controlled cantilever forces. It was possible to observe the formation of large surface areas covered with lipid in the presence of both HS- molecules. No I610 - PREPARATION AND STUDY OF IN VITRO differences between bilayers on DTT or DTE were observed. The measured BIOACTIVITY OF PMMA-BASED COMPOSITES FILLED height of the lipid coverage was ~6 nm, which corresponds to the formation of a DMPC bilayer. The membranes obtained are softly supported on the Au Márcio C. Fredel (UFSC), Mª Helena V. Fernandes (UA), Rui N. Correia treated substrates and can be easily scratched away by slightly increasing (UA), Nuno A. F. Almeida (UA), Poliana P. Lopes (UFSC). the force of AFM tip. This feature also opens the possibility of employing Bárbara J. M. Leite Ferreira (Universidade de Aveiro-UA) nanolithography methods with such systems. Additional electrochemical measurements were employed to evaluate the coverage of gold surfaces with DTE and DTE. The possible mechanisms of DTT and DTE binding onto To improve fixation of PMMA with the host bone and to prevent the aseptic loosening of the cement, various composites with bioactive materials have been developed and studied during the last years. The results obtained are encouraging and suggest that this materials are bioactive, form an apatite-like surface layer in vivo or in contact with SBF in vitro. The aim of this work was to produce bioactive PMMA-co-EHA/glass composites and consequently Gilberto Weissmüller (OFÍSICA/UFRJ), Gustavo Miranda Rocha follow the formation of the apatite-like layer on the surface of the prepared (OFÍSICA/UFRJ), Kevin Farah Buckley (IQ/UFRJ), Tereza Cristina Abreu Almeida (IQ/UFRJ), Carolina Chamusca Simões (IQ/UFRJ), Pierre Moté Esteves (IQ/UFRJ) and Vanessa L. Rodrigues Furtado (IQ/UFRJ) New poly(amidoamine) type membranes were prepared by reacting a I611 - GLIADIN FILMS CROSS-LINKED WITH 1-(3- generation 3 PAMAM dendrimer (32 amino groups at the surface) with DIMETHYLAMINOPROPYL)-3-ETHYLCARBODIIMIDE sebacoyl chloride using interface polymerization. Four different PAMAM: sebacoyl chloride molar ratios and two different concentrations of reagents solutions were tested. Products were characterized by IR spectroscopy and atomic force microscopy (AFM). These membranes can be easily modified Valdir Soldi (UFSC), Rosane M.D. Soares (UFSC) and Patricia Rayas for use in filtration, molecular diagnostics and controlled release of drugs.
Prolamins are the storage proteins of cereal seeds. In wheat seed the prolamins, namely gliadins, are a family of heterogeneous polypeptides. These proteins are of relevant industrial interest as revealed by the numerous patents that have been developed in recent years by the food and MONOLAYERS: SURFACE PRESSURE AND ELECTRONIC nonfood industries. In this work films were prepared by adding the protein into distilled water with different amount of plasticizer. Different proportions José Alberto Giacometti (FCT-UNESP), Carlos José Leopoldo of EDC/NHS were used. Gliadin films cross-linking with EDC/NHS was Constantino (FCT-UNESP) and Wilker Caetano (FCT-UNESP) successfully obtained. Moreover the changes in secondary structure of gliadin induced by cross-linking agent are still under investigation prior to The incorporation, binding moiety and molecular organization of Methylene obtain more detailed understanding in this issue. Blue has been investigated in Langmuir monolayers containing the phospholipids Cardiolipin and DOPC, used as very simple model systems to mimetize the mitochondrion membrane microenvironment, a likely target during the drug photosensitization process in photodynamic therapy (PDT). Cecilia Irene Vázquez (FCQ/UNC), Ma. Bernarda Quiroga Argañaraz (FCQ/UNC) and Gabriela Inés Lacconi (FCQ/UNC) The purpose of this work is the formation and characterization of hydrogen-terminated Si(111) surfaces functionalized with covalently attached alkyl chains and aromatic alkyl monolayers. The formation of the monolayers was performed using neat 1-octadecene or styrene, which react efficiently with the hydrogen-terminated Si(111) when heated at temperatures over 140ºC or under exposition to UV radiation. The electrochemical characterization of the functionalized Si surfaces is based on the layer ability to passivate the oxide formation and the structural characterization was performed by SPM.
ALUMINA-ZIRCONIA CERAMIC COMPOSITES WITH Roger R. Riehl (INPE), Maria do Carmo Andrade Nono (INPE), and Cosme Roberto Moreira Silva (IAE/CTA) Porous materials are of significant interest due to their wide application in catalysis, separation, biomaterials and other applications. Porosity and pore size distribution can be carefully controlled by the choice of organic composite and the amount added. There are many methods for obtaining of porous ceramics, in general consisting in adding organic particles, which volatilize during the first heat-up, to the ceramic matrix. The objective of this study was to produce ceramic composite nanostructure of alumina and yttria stabilized zirconia (Y-TZP) with micrometric pore sizes. PENETRATION OF POLY (D,L-LACTIDE) (PLA) AND POLY (D,L-LACTIDE)-POLY (ETHYLENE GLYCOL) (PLA- PEG) NANOCAPSULES CONTAINING ANTITUMORAL Elenara Lemos Senna (UFSC), Angela Machado de Campos (UFSC) Topical drug delivery to the eye is the most common treatment of ophthalmic diseases, and the cornea provides the dominant barrier to drug transport. The goals of this study were investigate the potential of these vehicles for delivering methotrexate across the cornea. Nanocapsules methotrexate in PLA and PEG-PLA polymer were prepared, characterized and investigated the potential of these carriers for delivering methotrexate across the cornea. The study retention corneal showed that amount of the drug keeped in the corneas was significantly increased by the nanocapsules of PLA (p<0.05) compared with nanocapsules of PLA-PEG after 2 hours of assay.

Source: http://www.sbpmat.org.br/5encontro/IndexPosterI.pdf