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- KOBAYASHI ZEIN DP -

Zein is a major component of corn proteins, called a prolamine soluble in aqueous ethanol.
Zein is extracted from gluten meal separated under corn starch production. The acquisition of
deodorized and decolourised zein is attained by Kobayashi’s method. It is called “KOBAYASHI
ZEIN DP”.
We supply “KOBAYASHI ZEIN DP” to be used for food processing, cosmetics use,
pharmaceutical applications and other various purposes with confidence because it is a natural
protein of corn.
APPEARANCE AND CHARACTERISTICS

White to pale yellow powder.
Insoluble in water, Hexane, Diethylether, Benzene, Toluene and Xylene.
soluble in aqueous ethanol (60-90% by weight), aqueous methanol (90% by weight), aqueous IPA
(60-90% by weight) and aqueous aceton (60-90% by weight).
SPECIFICATIONS


ADVANTAGES

FILM
Aqueous Ethanol solution of Zein is sprayed or spread in on materials, then dried. Zein film is formed on them. It is edible and resistant to water, acid and oil. Zein adheres tightly. For example, spread or sprayed Zein solution on timbers, etc. (dry surface) adheres them firmly. Zein is highly resistant to gastric acid and is digested by intestinal enzymes. SLOW RELESE Zein is combined and make tablet as matrix, then slow release tablet is obtained. By changing volume of Zein content, release time can be controlled. Zein has great anti-oxidation effect when Zein is in contact with oil. CBC (EUROPE) Ltd. Ufficio di Milano
Via E.Majorana 2 - 20054 - Nova Milanese (MI) Tel. +39 (0)362 365079 Fax. +39 (0)362 41273 E-Mail: chemical@cbc-europe.it www.cbc-europe.it - KOBAYASHI ZEIN DP -

GENERAL APPLICATIONS

EDIBLE COATING 1. prevention of oxidation of nuts
2. prevention of stickiness of chocolate
ENTERIC TABLET
MIXED FERTILIZER FOR FISH
OTHER YOUR OWN PURPOSE



CBC (EUROPE) Ltd. Ufficio di Milano
Via E.Majorana 2 - 20054 - Nova Milanese (MI) Tel. +39 (0)362 365079 Fax. +39 (0)362 41273 E-Mail: chemical@cbc-europe.it www.cbc-europe.it - KOBAYASHI ZEIN DP -

SPECIAL APPLICATIONS

FILM
1.
For spray coating: 8-15gr of “KOBAYASHI ZEIN DP” is dissolved into 100ml of aqueous ethanol (86% by weight). This solution is sprayed on materials and dried. How to dissolve of “KOBAYASHI ZEIN DP” Zein is dissolve in aqueous ethanol (86%) by weight) about 20%. Stir for 1 hour and reduce it with same aqueous ethanol to the concentration from 8 to 15% “KOBAYASHI ZEIN DP” should be added bit by bit into aqueous ethanol stirring vehemently.When a part of “KOBAYASHI ZEIN DP” forms a clod, it should be stirred for 1 hour. White precipitate may still appear in this solution after a few days. It would not avoid forming zein membrane but you may get rid of it filtering, etc. as you want. For pan work coating: 15-20gr of “KOBAYASHI ZEIN DP” is dissolved into 100ml of aqueous ethanol (86% by weight). This solution is sprayed on materials and dried. Addition of medium chain triglyceride etc. makes coating easy. CBC (EUROPE) Ltd. Ufficio di Milano
Via E.Majorana 2 - 20054 - Nova Milanese (MI) Tel. +39 (0)362 365079 Fax. +39 (0)362 41273 E-Mail: chemical@cbc-europe.it www.cbc-europe.it - KOBAYASHI ZEIN DP -
Cover tablet in a rotation pan with Zein solution.
After covering Zein solution, quickly spread it uniformary and stop rotation of the pan right
away.
Stationary drying should be performed in 5-10 minutes under cool wind condition(less than
10℃). If the pan is rotating in drying process, it may cause crumbing on the surface of tablet.
MCT (Medium Chain Triglycerides) help to protect table from gathering together during coating and give resistance to water. Free fatty acids (oleic acids etc.) give softness and transparency to the film. Glycerols give adhesiveness to surface of wax and solid fat. 10-25gr of “KOBAYASHI ZEIN DP” is dissolved into 100ml of aqueous ethanol (60-90% by weight). This solution is spread or sprayed on the surface you want to adhere. Addition “KOBAYASHI ZEIN DP” is mixed into powder material and aqueous ethanol (60-90% by weight) is added to this mixed powder. The mixture is kneaded and dried. Addition of plasticizer improves adhesion. CBC (EUROPE) Ltd. Ufficio di Milano
Via E.Majorana 2 - 20054 - Nova Milanese (MI) Tel. +39 (0)362 365079 Fax. +39 (0)362 41273 E-Mail: chemical@cbc-europe.it www.cbc-europe.it - KOBAYASHI ZEIN DP -
Placebo tablet : acid-red 52 in lactose (150mg/table) JP1 soln. + pepsin(2mg/ml)
JP2 soln. + chymotrypsin(0.2 mg/ml) 36-37°C
6.7-6.8
CBC (EUROPE) Ltd. Ufficio di Milano
Via E.Majorana 2 - 20054 - Nova Milanese (MI) Tel. +39 (0)362 365079 Fax. +39 (0)362 41273 E-Mail: chemical@cbc-europe.it www.cbc-europe.it - KOBAYASHI ZEIN DP -
JP1 soln. + pepsin(1.2)
1 tablet(200mg eq. as Aspirin)
CBC (EUROPE) Ltd. Ufficio di Milano
Via E.Majorana 2 - 20054 - Nova Milanese (MI) Tel. +39 (0)362 365079 Fax. +39 (0)362 41273 E-Mail: chemical@cbc-europe.it www.cbc-europe.it - KOBAYASHI ZEIN DP -
ANTI-OXYDATION Example of higher unsaturated fatty acid of fish oil Control corn starch : fatty acid of fish oil = 4 : 1 (Impregnate into corn starch ) Zein Zein : fatty acid of fish oil = 2 : 1(Drying after dissolve in ethanol) CBC (EUROPE) Ltd. Ufficio di Milano
Via E.Majorana 2 - 20054 - Nova Milanese (MI) Tel. +39 (0)362 365079 Fax. +39 (0)362 41273 E-Mail: chemical@cbc-europe.it www.cbc-europe.it

Source: http://www.cbc-europe.eu/images/stories/file/chemical/zeindep.pdf

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