Resistencia antimicrobiana y epidemiología entérica serovar Enteritidis aisladas en las provincias de Chaco y Corrientes (Argentina) biotipos, fagos y colicinas, pruebas de susceptibilidad antimicrobiana, perfil plasmídico, análisis del ADN cromosómico total por electroforesis en campo pulsátil (PFGE), estudio de secuencias IS200, análisis mediante PCR de las secuencias REP (repetitive
Md3.docPRODUCTION OF DRUG NANOPARTICLES OF
CONTROLLABLE SIZE USING SUPERCRITICAL
FLUID ANTISOLVENT TECHNIQUE WITH
ENHANCED MASS TRANSFER
Gupta R.B1, and Chattopadhyay P.*2
1-Auburn University, 2-Ferro Corporation. Ferro Corporation, 7500, E. Pleasant Valley Road, Independence, OH 44133. U.S.A. Email: firstname.lastname@example.org Fax: (216) 7506915
The use of supercritical fluids in the area of material processing and for particle
formation has been known for several years now. The advantages of supercritical fluid
processing include mild operating temperatures, production of solvent free particles and
easy micro encapsulation of particles. One of the attractive methods of particle
processing using supercritical fluid is the Supercritical Antisolvent (SAS) technique.
Although this technique has numerous advantages, it still cannot produce fine particles in
the sub-micron range (<300 nm) for soft materials. A significant improvement in the
SAS process has been proposed in this work, to obtain particles of controllable size that
are up to ten-fold smaller and have narrower size distributions. Like the conventional
SAS technique, the new technique Supercritical Antisolvent Precipitation with Enhanced
Mass Transfer (SAS-EM) also utilizes supercritical carbon dioxide as the antisolvent, but
in this case the solution jet is deflected by a surface vibrating at an ultrasonic frequency,
that atomizes the jet into much smaller droplets. Furthermore, the ultrasound field
generated by the vibrating surface enhances mass transfer and prevents agglomeration
through increased mixing. The particle size is easily controlled by varying the vibration
intensity of the deflecting surface, which can be adjusted by changing the power supplied
to the attached ultrasound transducer. This new technique is demonstrated by the
formation of nanoparticles of different pharmaceuticals such as lysozyme, tetracycline
Nanoparticles are important in developing delivery systems for controlled release of
drugs. These systems can improve the therapeutic efficacy of drugs, in-vitro and in-vivo
stability, bioavailability, targetability, and bio-distribution to reduce toxicity . The
delivery systems involving nanoparticles studied so far include, polymer nanoparticles
with the drug dispersed within the polymer matrix, drug nanoparticles coated with a
biodegradable polymer, polymer nanoparticles with the drug adsorbed on the surface, and
nanoparticle suspensions for poorly soluble drugs .
There have been several methods in the past for the manufacture of drug nanoparticles.
Some of the conventional techniques include spray drying and ultra fine milling [3, 4].
The major disadvantage of these techniques is that they produce particle having a broad
size distribution (0.5 - 25 µm) and only a small fraction of the particles produced are in
the nanometer range . Drug nanoparticles can also be prepared by precipitation process leading to the formation of hydrosols, but these methods have limitations due to difficulties in containing and controlling particle growth. In recent years however, supercritical fluid technologies such as Rapid Expansion of Supercritical Solutions (RESS) [6-8] and Supercritical Antisolvent (SAS) [9-11] precipitation have emerged as attractive methods for drug and biological particle formation. Some of the advantages of these techniques include mild operating temperatures and absence of residual solvent. The particles obtained by these techniques are 0.7 - 5.0 µm in size and have a narrow size distribution. Although these techniques are getting increasingly popular, in most cases they still do not produce particles predominantly in the nanometer range (< 300 nm) necessary for drug targeting and controlled release. In the SAS process the operating temperature, pressure and concentration of the injecting solution have so far been investigated as size control parameters, but none of these parameters have been found to produce a significant decrease in the particle size over a wide range. Again the extensive application of the RESS technique is limited by solubility limit of the solid being precipitated in supercritical fluid. Keeping this in mind, we have proposed a technique that can be used to manufacture particles in the nanometer range having a very narrow size distribution. This new technique, supercritical antisolvent with enhanced mass transfer (SAS-EM) , is a modification of the conventional SAS process and overcomes the currently existing limitations of the SAS process. Like the SAS process, the new technique also uses supercritical CO2 as an antisolvent. The modification in the new technique is that it utilizes a surface, vibrating at an ultrasonic frequency to atomize the solution jet into micro-droplets. Moreover, the ultrasound field generated by the horn surface provides a velocity component in the y-direction (direction normal to the vibrating surface) that greatly enhances turbulence and mixing within the supercritical phase resulting in high mass transfer between the solution and the antisolvent. The combined effect of fast rate of mixing between the antisolvent and the solution, and reduction of solution droplet size due to atomization, provides particles approximately ten-fold smaller than those obtained from the conventional SAS process. The use of high energy sonic waves for particle precipitation using supercritical fluids has also been suggested Subramaniam et al. (1997) . In their case a specialized nozzle, of the type commercialized by Sonomist, Model 600-1 was used to generate and focus the high frequency sonic waves for atomization. Randolph et al. (1993)  also employed a specialized nozzle during the precipitation of poly (L-lactic acid) particles using the SAS technique. The nozzle in their case was a Sonotek atomizer having a capillary tube, which was vibrated at 120 kHz to produce a narrow cylindrical spray. The proposed technique also uses high frequency sound for atomization but the atomization process is brought about by introducing the solution on a vibrating surface in the form of a thin liquid film. No specialized nozzle has been used in this technique. In this paper we demonstrate the application of SAS-EM technique for the formation of tetracycline, griseofulvin, and lysozyme micro and nanoparticles required for drug delivery and other pharmaceutical purposes.
A, B - Nitrogen and carbon dioxide cylinders respectively.
C - Hand pump to fill carbon dioxide into the precipitation cell.
V, P- Control valves and Pressure gauges respectively.
S - Solution injection device.
R - Precipitation cell maintained at high pressure.
UP - Ultrasonic processor.
H - Titanium Horn
Figure 1. Schematic diagram of the SAS-EM apparatus
A schematic representation of the apparatus is shown in Figure 1. The main component
of the apparatus consists of a high-pressure ultrasound precipitation cell (R)
approximately 80 cm3 in volume. A titanium horn (Sonics and Materials, Inc.) having a
tip 1.25 cm in diameter is attached to the precipitation cell to provide the ultrasonic field
and the vibrating surface necessary for atomization. The vibrations of the horn surface
are generated by a 600 W (maximum power), 20 kHz ultrasonic processor (Ace Glass,
Inc.). The ultrasonic processor is designed to deliver constant amplitude. The amplitude
of vibration of the ultrasonic horn is directly proportional to the total input power and can
be directly controlled by adjusting the power supplied to the ultrasound transducer. A
collection plate is placed inside the precipitation chamber for collecting the particles.
High pressure inside the chamber is generated using a HIP hand pump (C). Valves V1
and V2 are used to fill up the HIP hand pump with fresh CO2. Temperature inside the
precipitation cell is maintained by placing it in a constant temperature water bath. The
solution containing the solid to be precipitated is injected inside the precipitation cell
using a "solution injection device" (S), which consists of a stainless steel cylinder
containing a piston. The piston divides the cylinder into two chambers. The antibiotic
solution is placed inside one of these chambers and is delivered into the cell by using
pressurized nitrogen in the other chamber. The device S is connected to the vessel by
means of a 75-µm i.d. fused quartz capillary tube. A pressure drop of 28 bar is
maintained across the capillary tube and the device S in order to spray the solution inside
the precipitation chamber. The capillary tube is placed at an angle of 40o with respect to
the horn surface in a manner such that the capillary opening touches the horn surface.
Supercritical CO2 is fed inside the precipitation chamber through the inlet port located at
the bottom of the vessel. Valve V1 is used to control the flow of Supercritical CO2 into
the high-pressure chamber. Pressure inside the chamber is measured using a pressure
gauge P1. The outlet port is located on top of the precipitation chamber and valve V4 is
used to control the depressurization process. The pressure difference across the capillary
and the solution injection device is measured using the pressure gauge P2 and P1.
All the precipitation experiments were carried out in the batch mode and in an identical
manner. First ultrasonic precipitation cell was filled with carbon dioxide up to desired
operating pressure. The temperature inside the chamber was maintained using a water
bath. Approximately 1.5-2.0 g of solvent containing the drug (5 mg/ml) was then loaded
into the "solution injection device" (S). The ultrasonic horn inside the cell was then
switched on at the desired vibration amplitude by adjusting the input power, and the
solution was introduced inside the precipitation chamber through the 75 µm capillary
tube, placed against the horn surface at an angle of 40o. As soon as the solution jet was
introduced into the precipitation chamber particles were precipitated in the nanometer
range due to enhanced mass transfer between the solvent and the supercritical fluid.
Motion between the particles inside the chamber was increased due to the ultrasonic field
generated by the horn surface, which prevented agglomeration. The injection process
was typically completed in 2 - 3 minutes and the power supply to the ultrasonic horn was
turned off thereafter.
Next was the washing step in which residual solvent, left dissolved in supercritical CO2
was removed by continuously purging the precipitation chamber with fresh CO2. The
complete cleaning process required approximately 7-8 times the vessel volume of fresh
CO2. The precipitation cell was then allowed to slowly depressurize till it reached
ambient pressure. The chamber was then opened and the collection plate was removed
and taken for particle analysis.
Results of the precipitation experiments conducted using the SASEM technique at 96.5
bar, 37 oC and at different values of ultrasound power supply, for various pharmaceutical
compounds, have been shown below. The experiments clearly illustrate that with
increasing power supply to the transducer there is a decrease in the size of the
Mean size of
We have demonstrated the precipitation of tetracycline and lysozyme nanoparticles
having sizes as low as 125 nm and190 nm using the SAS-EM technique. The particles
obtained by this process are 5-8 times smaller than those obtained from the conventional
SAS process. We have also been successful in reducing the size of griseofulvin particles
from several millimeter long needles obtained in the conventional SAS process to 0.2-2.0
µm size particles. Particle sizes in the SAS-EM technique are easily controlled by
adjusting the power supplied to the transducer. At higher ultrasound power, apart from a
decrease in the particle size, the standard deviation in particle size in most cases is also
much lower. Thus, particles having a narrower size distribution are obtained using the
1. LABHASETWAR, V. Pharm. News. 1997, 4(6), 28.
2. LANGER, R. Science. 1990, 249, 1527.
3. NASS, R. Lieberman, H.A.; Rieger, M.; Banker, B., Eds.; Marcel Dekker: New
4. HIXON, L., PRIOR, M., PREM, H., VAN CLEEF, J. Sizing Chem. Eng. 1990,
5. REVERCHON, E; DELLA PORTA, G. Powder Technology. 1999, 106, 23.
6. REVERCHON, E.; DELLA PORTA, G; TROLIO; PALLADO, P.; STASSI, A.
Ind. Eng. Chem. Res. 1995, 34 (No. 11), 4087.
7. HELFGEN, B.; HILS, P.; HOLZKNECHT, C.; TURK, M.; SCHABER, K. J. Aerosol Sci. 2001, 32(3), 295.
8. CHAROENCHAITRAKOOL, M.; DEHGHANI, F.; FOSTER, N. R.; CHAN, H. K. Ind. Eng. Chem. Res. 2000, 39(12), 4794.
9. WINTERS, M. A. KNUTSON, B. L.; DEBENEDETTI, P.G.; SPARKS, H. G. .; PRZYBCIEN, T. M.; STEVENSON, C. L.; PRESTRELSKI, J. S. Journal of
Pharmaceutical Sciences 1996, 85, 586.
10. YEO, S.-F., LIM, G.-B., DEBENEDETTI, P.G., BERNSTEIN, H. Biotechnology and Bioengineering, 1993, 41, 341.
11. REVERCHON E. Journal of Supercritical Fluids. 1999, 15, 1.
12. GUPTA, R. B., CHATTOPADHYAY, P. US Provisional Patent 60/206,644,
13. SUBRAMANIAM, B.; SAIM, S.; RAJEWSKI,R.A.; STELLA, V. US patent 14. RANDOLPH, T.W., RANDOLPH, A.D. MEBES, M., AND YEUNG, S., Biotechnol. Prog., 9, 429 (1993).
AP Statistics: Chapter 12.2: Inference for TWO ProportionsIn two-sample problems, we want to compare responses ot two independent samples. In chapter 11, we compare two means using a two-sample t procedures. In chapter 15, we will compare two standard deviations using an F statistic. In a two-sample proportion problem (this section) we want to compare two populations or the responses of two